EGFL7-overexpressing epidermal stem cells promotes fibroblast proliferation and migration via mediating cell adhesion and strengthening cytoskeleton.
EGFL7-overexpressing epidermal stem cells promotes fibroblast proliferation and migration via mediating cell adhesion and strengthening cytoskeleton.
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EGFL7 过表达表皮干细胞通过介导细胞粘附和强化细胞骨架促进成纤维细胞增殖和迁移。
DOI：
10.1007/s11010-016-2812-0
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Xie Ju-Lin
中科院分区：
文献类型：
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作者：
Yang Rong-Hua;Qi Shao-Hai;Ruan Shu-Bin;Lin Ze-Peng;Lin Yan;Zhang Feng-Gang;Chen Xiao-Dong;Xie Ju-Lin
表皮生长因子（EGF）家族成员介导广泛的生物活动，包括细胞增殖和迁移。越来越多的证据表明，EGF通过刺激成纤维细胞运动在伤口愈合过程中发挥重要作用。本研究的目的是观察过表达egf样结构域7 （EGFL7）的表皮干细胞（EGFL7- escs）是否会促进成纤维细胞的增殖和迁移。我们发现EGFL7- escs中EGFL7 mRNA和蛋白表达水平显著升高。与ESCs CM或载体ESCs CM相比，EGFL7-ESCs的条件培养基（CM）中EGFL7蛋白的表达显著升高。EGFL7-ESCs cm处理的成纤维细胞的细胞计数和细胞活力也显著高于对照组。此外，与对照组相比，EGFL7-ESCs cm处理的成纤维细胞表现出更高的迁移。此外，在EGFL7-ESCs cm处理的成纤维细胞中，β1-整合素、β-微管蛋白、β-肌动蛋白和Vimentin的表达增加，而E-cadherin的表达减少。这些结果表明，EGFL7-ESCs通过增强细胞粘附、增强细胞骨架和减少细胞间聚集来促进成纤维细胞迁移。这些发现表明，EGFL7-ESCs对成纤维细胞增殖和迁移的刺激作用可能为伤口愈合提供了有用的策略。
Epidermal growth factor (EGF)-like family members mediate a wide range of biological activities including cell proliferation and migration. Increasing evidence indicated that EGF plays an important role in the process of wound healing by stimulating fibroblast motility. The aim of this study was to see whether EGF-like domain 7 (EGFL7)-overexpressing epidermal stem cells (EGFL7-ESCs) would promote fibroblast proliferation and migration. We found that mRNA and protein levels of EGFL7 expression were significantly increased in EGFL7-ESCs. The protein expression of EGFL7 was significantly elevated in conditioned media (CM) of EGFL7-ESCs compared to ESCs CM or vector-ESCs CM. The cell count and cell viability of EGFL7-ESCs CM-treated fibroblasts were also significantly increased compared to control. In addition, EGFL7-ESCs CM-treated fibroblasts showed elevated migration compared with control. Moreover, the expressions of β1-integrin, β-tubulin, β-actin, and Vimentin were increased, while that of E-cadherin was decreased in EGFL7-ESCs CM-treated fibroblasts. These results indicate that EGFL7-ESCs contribute towards promoting fibroblast migration through enhancing cell adhesion, strengthening cytoskeleton, and reducing intercellular aggregation. These findings suggest that the stimulating effect of EGFL7-ESCs on fibroblast proliferation and migration may provide a useful strategy for wound healing.