Comparative proteomics analysis reveals the mechanism of pre-harvest seed deterioration of soybean under high temperature and humidity stress.
Comparative proteomics analysis reveals the mechanism of pre-harvest seed deterioration of soybean under high temperature and humidity stress.
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DOI：
10.1016/j.jprot.2012.01.007
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发表时间：
2012-04
影响因子：
3.3
通讯作者：
Liqun Wang;Hao Ma;Liru Song;Yingjie Shu;Weihong Gu
中科院分区：
文献类型：
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作者：
Liqun Wang;Hao Ma;Liru Song;Yingjie Shu;Weihong Gu
大豆种子在田间发育和成熟过程中受到高温高湿胁迫，易导致种子采前劣变。然而，蛋白质及其参与的途径如何系统地导致大豆种子的劣变仍然没有得到很大的理解。为了揭示这一点，我们采用双向电泳技术，比较了不同HTH胁迫时间点（24、96和168 h）下，一个对采前种子劣变敏感的大豆品种与其相应对照的发育种子（R7期）蛋白质组组成。通过MALDI-TOF MS分析，共发现42个差异表达蛋白点，并与31个不同的蛋白质种类相匹配。这些蛋白质参与了13个细胞反应和代谢过程，包括碳水化合物代谢、信号转导、蛋白质生物合成、光合作用、蛋白质折叠和组装、能量途径、细胞拯救和防御、细胞周期、氮代谢、脂质代谢、氨基酸代谢、转录调控和次生代谢物生物合成。基于这些蛋白质的功能和参与途径，结合超微结构、理化和代谢组学数据，提出了种子采前劣变的机理。这种机制使我们能够进一步了解HTH胁迫下发育种子中细胞活动的可能管理策略，并为作物发育种子中HTH胁迫反应提供了新的见解。
High temperature and humidity (HTH) stress during soybean seed development and maturity in the field easily leads seed to pre-harvest deterioration. However, how proteins and their involved pathways in developing soybean seed systematically cause deterioration is still not largely understood. To reveal it, we compared the proteome composition of developing seed (R7period) of a pre-harvest seed deterioration sensitive soybean cultivar at different HTH stress time points (24, 96 and 168h) with their corresponding controls by 2-DE. 42 protein spots were found to be differentially expressed and successfully identified by MALDI-TOF MS to match 31 diverse protein species. These proteins were involved in 13 cellular responses and metabolic processes including carbohydrate metabolism, signal transduction, protein biosynthesis, photosynthesis, protein folding and assembly, energy pathway, cell rescue and defense, cell cycle, nitrogen metabolism, lipid metabolism, amino acid metabolism, transcription regulation, and secondary metabolite biosynthesis. Based on these proteins' functions and involved pathways, together with ultrastructural, physical and chemical, and metabolomic data, a pre-harvest seed deterioration mechanism was proposed. Such a mechanism allows us to further understand the possible management strategy of cellular activities occurring in the HTH-stressed developing seeds and provides new insights into the HTH stress responses in crop developing seeds.