[Two-dimensional gel electrophoresis map of serum proteins in patients with chronic Keshan disease].
[Two-dimensional gel electrophoresis map of serum proteins in patients with chronic Keshan disease].
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发表时间：
2013-05
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通讯作者：
Yu-xiao Sun;Yanyan Zhu;Jianhong Zhu;Xiao‐lin Niu;Chao Yan;Guang Yang;Lin Lin-Lin
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作者：
Yu-xiao Sun;Yanyan Zhu;Jianhong Zhu;Xiao‐lin Niu;Chao Yan;Guang Yang;Lin Lin-Lin
目的鉴定克山病(KD)患者血清中表达的差异蛋白。方法采用双向凝胶电泳法(2-DE)对慢性川崎病患者和健康对照血清进行蛋白质分离。凝胶用银染，UMAX扫描仪扫描。采用ImageMaster 2D软件对数据进行统计分析。检索ExPASy Swiss-2DPAGE数据库，鉴定KD相关蛋白。结果建立了KD患者和健康对照血清稳定的双向凝胶电泳图。KD患者和健康对照组分别观察到808个和814个蛋白点。两个图谱有96.5475%的同源性蛋白质斑点和44个差异表达蛋白质斑点。11个蛋白质斑点仅在KD患者中表达，12个蛋白质斑点仅在健康对照中出现。两组均有约21种蛋白质表达，但数量不同(KD患者中有14种蛋白质高表达3倍以上，7种蛋白质低表达3倍以上，P<；0.01)。在与ExPASy-Swiss-2DPAGE数据库匹配的353个蛋白质点中，发现在KD患者中出现的1177个蛋白质与P02774 2-D0004T6最接近，称为维生素D结合蛋白(VDBP)。结论KD患者血清蛋白表达与正常人有显著差异。VDBP可能通过炎症免疫反应在心肌损伤中发挥作用。
OBJECTIVE To identify the different serum proteins expressed in patients with Keshan disease (KD). METHODS Two-dimensional gel electrophoresis (2-DE) was performed with serum samples from the patients with chronic KD and healthy controls to separate serum proteins. The gels were stained by sliver and scanned by Umax scanner. The data were analyzed by ImageMaster 2D software. KD related proteins were identified through searching the ExPASy SWISS-2DPAGE database. RESULTS Stable two-dimensional gel electrophoresis maps were established for serum samples of KD patients and healthy controls. A total of 808 and 814 protein spots were observed in KD patients and healthy controls, respectively. The two maps had 96.5475% identical protein spots and 44 differentially expressed protein spots. Eleven protein spots were expressed exclusively in KD patients and 12 protein spots only appeared in healthy controls. About 21 proteins were expressed in both groups but varied in quantities (14 proteins were over-expressed by more than 3 times and 7 proteins were under-expressed by more than 3 times in KD patients, P < 0.01). Among the 353 protein spots matched with the ExPASy-SWISS-2DPAGE databank, No. 1177 protein appeared in the KD patient was found to have the closest match with P02774 2-D0004T6 known as vitamin D binding protein (VDBP). CONCLUSION There is a significant difference in serum protein expression between KD patients and normal people. VDBP might play a role in cardiac muscle damage via inflammatory immune reactions.