Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions
Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions
复制标题
DOI：
10.1016/s0142-9612(00)00009-0
复制
发表时间：
2000-06-01
期刊：
影响因子：
14
通讯作者：
Svorcík, V
中科院分区：
文献类型：
--
作者：
Bacáková, L;Mares, V;Svorcík, V
将来自牛肺动脉的内皮细胞（细胞系CPAE，CCL 209，美国组织培养物保藏中心，Rockville，MD，USA）在原始或氟离子辐照的聚苯乙烯（5 × 10（12）或5 × 10（14）F离子/cm（2），150 keV）上培养。在接种后24小时间隔，粘附到离子改性的聚苯乙烯的细胞数量显著高于未改性的材料（在用较低和较高离子剂量照射的聚苯乙烯培养物中分别为+20和+58%）。在第7天，在照射的基板上培养的群体生长到更高的密度，超过控制在较低和较高的离子剂量的69和180%，分别。离子注入样品上的细胞也更大（在较低和较高离子剂量下分别为+70-95%和+90-99%），并且含有更多蛋白质（在两种离子剂量下均为+16%）。如ELISA所示，经较高离子剂量照射的聚苯乙烯增强了细胞骨架蛋白波形蛋白（+65%）和粘着斑蛋白塔林（+15%）的表达。整合素α（5）β（1）（VLA-5）的含量，纤连蛋白受体，在较低和较高的离子剂量（+22和+57%）增加。与此相反，ICAM-1和黏着斑蛋白的含量在原始和离子辐照的生长基质上生长的细胞中相似。此外，在两个离子剂量组中，VCAM-1和ELAM-1的表达降低了11-14%。目前的研究表明，离子注入聚合物改善了内皮细胞的粘附和生长，而不提高免疫球蛋白和选择素类型的粘附分子的表达。这种表面改性应促进内皮细胞对人造血管假体的定殖，并使其不易受到受体免疫系统细胞的攻击。(C)2000爱思唯尔科技有限公司版权所有。
Endothelial cells derived from the bovine pulmonary artery (line CPAE, CCL 209, American Tissue Culture Collection, Rockville, MD, USA) were cultured on pristine or fluorine ion-irradiated polystyrene (5 x 10(12) or 5 x 10(14) F ions/cm(2), 150 keV). At 24-h post-seeding interval, the number of cells which adhered to the ion-modified polystyrene was significantly higher than on the unmodified material (+20 and +58% in cultures with the polystyrene irradiated by lower and higher ion doses, respectively). On day 7, the populations cultured on the irradiated substrates grew to higher densities, exceeding the controls at the lower and higher ion doses by 69 and 180%, respectively. The cells on ion-implanted samples were also larger (+70-95% and +90-99% at the lower and higher ion doses, respectively) and contained more protein (+16% at both ion doses). As was shown by ELISA, the polystyrene irradiated by the higher ion dose enhanced the expression of a cytoskeletal protein, vimentin (+65%) and protein of focal adhesion plaques, talin (+15%). The content of integrin alpha(5)beta(1) (VLA-5), receptor for fibronectin, was increased at both lower and higher ion doses (+22 and +57%). In contrast to this, the content of ICAM-1 and vinculin was similar in cells grown on both pristine and ion-irradiated growth substrates. Moreover, the expression of VCAM-1 and ELAM-1 was lower by 11-14% in both ion dose groups. The present study has shown that ion implantation of polymers improves the adhesion and growth of endothelial cells without elevating the expression of immunoglobulin and selectin types of adhesion molecules. This surface modification should promote colonization of an artificial vascular prosthesis by endothelial cells and make it less vulnerable by immune system cells of the recipient. (C) 2000 Elsevier Science Ltd. All rights reserved.