Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)
Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)
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DOI：
10.1039/b920590j
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Davalos, Rafael V.
中科院分区：
文献类型：
--
作者：
Shafiee, Hadi;Sano, Michael B.;Davalos, Rafael V.
非接触式介电电泳（cDEP）是最近发展起来的一种细胞操作方法，其中电极与样品物理隔离。在这里，我们提出了两个微流控装置能够选择性地分离活的人类白血病细胞从死细胞利用他们的电气签名。通过数值模拟和实验验证了不同电压和频率对电场梯度和器件性能的影响。利用这些原型装置，我们能够在0.2-0.5 mm s（-1）下实现大于95%的去除效率，并且在活细胞和死细胞之间具有100%的选择性。与富集相结合，cDEP可以与其他技术相结合，以产生能够感测、分选和识别稀有细胞的全自动芯片实验室系统。
Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95% removal efficiency at 0.2-0.5 mm s(-1) with 100% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells.