Genome sequences of two multidrug-resistant Escherichia coli strains MEZEC8 and MEZEC10 isolated from livestock in South Africa.
Genome sequences of two multidrug-resistant Escherichia coli strains MEZEC8 and MEZEC10 isolated from livestock in South Africa.
复制标题
从南非牲畜中分离出的两种多重耐药大肠杆菌菌株 MEZEC8 和 MEZEC10 的基因组序列。
DOI：
10.1016/j.jgar.2020.11.008
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
Järhult,JosefD
中科院分区：
文献类型：
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作者：
ElZowalaty,MohamedE;Hickman,RachelA;Gambushe,SydneyM;Zishiri,OliverT;ElZowalaty,AhmedE;Järhult,JosefD
目的家畜大肠杆菌耐药性的出现是一个严重的公共卫生问题。在这里，我们报告了两个多重耐药的家畜相关的基因组序列草案。从南非绵羊分离的大肠杆菌MEZEC 8和MEZEC 10。使用Illumina MiSeq平台对大肠杆菌菌株MEZEC 8和MEZEC 10进行测序。修剪生成的读段并重新组装。对组装的重叠群进行抗生素耐药基因、染色体突变和染色体外质粒分析，并通过多位点序列分型（MLST）确定序列类型（ST）。将MEZEC 8和MEZEC 10与其他已发表的E.大肠杆菌，E.从NCBI的Sequence Read Archive和所有的序列文件进行了相同的处理，以产生一个核心的基因组自举最大似然系统发育树.ResultsAntimicrobial resistance genes被检测到MEZEC 8和MEZEC 10赋予四环素和大环内酯类抗生素抗性。MEZEC 10含有两个染色体外质粒（pO 111和Incl 2），而MEZEC 8不含有任何染色体外质粒。MEZEC 8株属于血清型H25：O9和ST 58，MEZEC 10株属于血清型H49：O8和ST 1844。大肠杆菌MEZEC 8和MEZEC 10可作为耐药畜禽大肠杆菌分子流行病学研究的参考菌株。非洲大肠杆菌。此外，这项研究允许深入分析基因组结构，并将提供有价值的信息，使我们能够了解畜牧相关的耐药性E。杆菌
ObjectivesThe emergence of antimicrobial-resistant livestock-associatedEscherichia colirepresents a great public health concern. Here we report the draft genome sequences of two multidrug-resistant livestock-associatedE. colistrains MEZEC8 and MEZEC10 isolated from sheep in South Africa.MethodsGenomic DNA ofE. colistrains MEZEC8 and MEZEC10 was sequenced using an Illumina MiSeq platform. Generated reads were trimmed and de novo assembled. The assembled contigs were analysed for antimicrobial resistance genes, chromosomal mutations and extrachromosomal plasmids, and the sequence type (ST) was determined by multilocus sequence typing (MLST). To compare strains MEZEC8 and MEZEC10 with other previously published sequences ofE. colistrains, raw read sequences ofE. colifrom livestock were downloaded from the NCBI’s Sequence Read Archive and all sequence files were treated identically to generate a core genome bootstrapped maximum likelihood phylogenetic tree.ResultsAntimicrobial resistance genes were detected in MEZEC8 and MEZEC10 conferring resistance to tetracycline and macrolides. MEZEC10 harboured two extrachromosomal plasmids (pO111 and Incl2), while MEZEC8 did not contain any extrachromosomal plasmids. Strain MEZEC8 belonged to serotype H25:O9 and ST58, whereas strain MEZEC10 belonged to serotype H49:O8 and ST1844.ConclusionThe genome sequences ofE. colistrains MEZEC8 and MEZEC10 will serve as a reference point for molecular epidemiological studies of antimicrobial-resistant livestock-associatedE. coliin Africa. In addition, this study allows in-depth analysis of genomic structure and will provide valuable information enabling us understand the antimicrobial resistance of livestock-associatedE. coli.