Morphological Changes in Vesicles and Release of an Encapsulated Compound Triggered by a Photoresponsive Malachite Green Leuconitrile Derivative
Morphological Changes in Vesicles and Release of an Encapsulated Compound Triggered by a Photoresponsive Malachite Green Leuconitrile Derivative
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DOI：
10.1021/la904190c
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发表时间：
2010-04-20
期刊：
影响因子：
3.9
通讯作者：
Kimura, Keiichi
中科院分区：
文献类型：
--
作者：
Uda, Ryoko M.;Hiraishi, Eri;Kimura, Keiichi
磷脂酰胆碱囊泡中的光诱导形态学变化由溶解在磷脂膜中的孔雀石绿色亮腈衍生物触发，并且在孔雀石绿色衍生物/脂质比< 5摩尔%时观察到。该孔雀石绿色衍生物是一种光响应性化合物，其通过UV照射进行电离以在分子上提供正电荷。孔雀石绿色衍生物在光化学电离时表现出两亲性，而在黑暗条件下表现为亲脂性化合物。冷冻透射电子显微镜用于确定囊泡形态。利用动态光散射和荧光共振能量转移研究了孔雀石绿绿色衍生物对囊泡的影响。照射含有孔雀石绿色衍生物的囊泡诱导非球形囊泡形态。囊泡的融合和膜溶解，这取决于条件。此外，照射孔雀石绿色衍生物诱导囊泡包封的化合物的释放。
Photoinduced morphological changes in phosphatidylcholine vesicles are triggered by a Malachite Green leuconitrile derivative dissolved in the lipidic membrane, and are observed at Malachite Green derivative/lipid ratios < 5 mol%. This Malachite Green derivative is a photoresponsive compound that undergoes ionization to afford a positive charge on the molecule by UV irradiation. The Malachite Green derivative exhibits amphiphilicity when ionized photochemically, whereas it behaves as a lipophilic compound under dark conditions. Cryo-transmission electron microscopy was used to determine vesicle morphology. The effects of the Malachite Green derivative on vesicles were studied by dynamic light scattering and fluorescence resonance energy transfer. Irradiation of vesicles containing the Malachite Green derivative induces nonspherical vesicle morphology. fusion of vesicles, and membrane solubilization, depending on conditions. Furthermore, irradiation of the Malachite Green derivative induces the release of a vesicle-encapsulated compound.