HTLV‐II Non‐integrated Malignant Lymphoma Induction in Japanese White Rabbits Following Intravenous Inoculation of HTLV‐II‐infected Simian Leukocyte Cell Line (Si‐IIA)
HTLV‐II Non‐integrated Malignant Lymphoma Induction in Japanese White Rabbits Following Intravenous Inoculation of HTLV‐II‐infected Simian Leukocyte Cell Line (Si‐IIA)
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静脉接种 HTLV-II 感染的猿白细胞系 (Si-IIA) 后日本白兔体内 HTLV-II 非整合性恶性淋巴瘤诱导
DOI：
10.1111/j.1349-7006.1994.tb02952.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
T. Akagi
中科院分区：
文献类型：
--
作者：
K. Hayashi;N. Ohara;T. Koirala;H. Ino;Hong Li Chen;N. Teramoto;E. Kondo;T. Yoshino;Kiyoshi Takahashi;M. Yamada;N. Tomita;K. Miyamoto;K. Fujimoto;Y. Yoshikawa;T. Akagi
报告了一种来源于产生HTLV-II-的猴（食蟹猴）白细胞系（Si-IIA）的未知因子在家兔中诱导淋巴瘤。静脉接种Si‐IIA细胞的17只雄性日本白色家兔中有13只（76%）在接种后62 - 167天发生恶性淋巴瘤，包括霍奇金样淋巴瘤。历史上，在许多器官中存在大细胞型或混合型淋巴瘤细胞的广泛弥漫性或结节性浸润，通常累及脾、肝、淋巴结和肾，较少累及胸腺、骨髓、肺、心脏、皮肤和胃肠道。还在两只家兔中观察到霍奇金样淋巴瘤。从荷瘤兔建立的5个细胞系的染色体分析显示了雄兔核型。这些肿瘤细胞的免疫表型通常为T细胞（CD 5+或，r RT 1+，RT 2+或-，CD 45+，CD 4-，RABELA-和MHC II类-DQ+），但仅表达CD 45的霍奇金样淋巴瘤细胞除外。然而，通过聚合酶链反应或Southern印迹分析，在肿瘤组织或任何兔细胞系中均不能证明HTLV-II前病毒基因组的整合。此外，接种HTLV-IIC、MOT（其他产生HTLV-II的人细胞系）或TALL-1（对照）未诱导淋巴瘤。注射来自Si-IIA培养物的无细胞团块的4只家兔中有2只死于恶性淋巴瘤（15 - 20天）。接种了5个经辐照的兔细胞系，但在第27天时，3只兔中只有1只（Ra-SLN）诱导了淋巴瘤。通过免疫荧光检测，在Si-IIA细胞中未检出松鼠猴疱疹病毒和猴疱疹病毒（猴致癌病毒）。这些数据表明，家兔中淋巴瘤诱导率高可能不仅是由HTLV-II或众所周知的猿猴致癌病毒引起的，而是由来源于Si-IIA或HTLV-IIA的未知过客因子引起的，其中Si-IIA是用HTLV-IIA建立的。
Lymphoma induction in rabbits by an unknown factor derived from an HTLV‐II‐producing simian (Cynomolgus) leukocyte cell line (Si‐IIA) is reported. Thirteen of 17 male Japanese white rabbits (76%) inoculated intravenously with Si‐IIA cells developed malignant lymphoma including Hodgkin‐like lymphoma between 62 and 167 days after inoculation. Historically, there was extensive diffuse or nodular infiltration of either large cell type or mixed type lymphoma cells in many organs, frequently involving the spleen, liver, lymph nodes and kidneys, and less frequently the thymus, bone marrow, lungs, heart, skin and gastrointestinal tract. Hodgkin‐like lymphoma was also observed in two rabbits. Chromosomal analysis of five cell lines established from tumor‐bearing rabbits revealed the male rabbit karyotype. The immunophenotype of these tumor cells was usually T‐cell (CD5+or, r RT1+, RT2+or‐, CD45+, CD4−, RABELA− and MHC class II‐DQ+) except for Hodgkin‐like lymphoma cells which expressed only CD45. However, integration of the HTLV‐II provirus genome could not be demonstrated in the tumor tissues or any of the rabbit cell lines by polymerase chain reaction or Southern blot analysis. Moreover, no lymphoma was induced by inoculation of HTLV‐IIC, MOT (other HTLV‐II‐producing human cell lines) or TALL‐1 (control). Two of four rabbits injected with cell‐free pellets from Si‐IIA cultures died of malignant lymphoma (15‐20 days). Five irradiated rabbit cell lines were inoculated but only one (Ra‐SLN) induced lymphoma in 1 of 3 rabbits at 27 days. Neither Herpesvirus saimiri nor Herpesvirus ateles (simian oncogenic viruses) was detected in Si‐IIA cells by immunofluorescence testing. These data suggest that the high rate of lymphoma induction in rabbits may be caused not by only HTLV‐II or well known simian oncogenic viruses, but rather by an unknown passenger agent derived from Si‐IIA or HTLV‐IIA, with which Si‐IIA was established.
在患有大颗粒淋巴细胞白血病的患者中检测人类 T 细胞白血病/淋巴瘤病毒 II 型。
影响因子：
20.3
作者：
LoughranJr,TP;Coyle,T;Sherman,MP;Starkebaum,G;Ehrlich,GD;Ruscetti,FW;Poiesz,BJ
通讯作者：
Poiesz,BJ
与蕈样肉芽肿相关的人类嗜淋巴细胞逆转录病毒：证据表明人类 T 细胞嗜淋巴细胞病毒 II 型 (HTLV-II) 以及 HTLV-I 可能在该疾病中发挥作用。
影响因子：
20.3
作者：
Zucker-Franklin,D;Hooper,WC;Evatt,BL
通讯作者：
Evatt,BL