A 128-base-pair sequence containing the pac1 and a presumed cryptic pac2 sequence includes cis elements sufficient to mediate efficient genome maturation of human cytomegalovirus.
A 128-base-pair sequence containing the pac1 and a presumed cryptic pac2 sequence includes cis elements sufficient to mediate efficient genome maturation of human cytomegalovirus.
复制标题
包含 pac1 的 128 个碱基对序列和推测的神秘 pac2 序列包含足以介导人巨细胞病毒有效基因组成熟的顺式元件。
DOI：
10.1128/jvi.02307-10
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发表时间：
2011
影响因子：
5.4
通讯作者：
McVoy,MichaelA
中科院分区：
文献类型：
--
作者：
Wang,JianBen;McVoy,MichaelA
Herpesvirus DNA replication proceeds via concatemeric replicative intermediates that are comprised of head-to-tail linked genomes. Genome maturation is carried out by the terminase, an enzyme complex that mediates both the insertion of concatemer DNA into capsids and its subsequent cleavage to release genomes within these capsids. This cleavage is sequence specific, but the governingcis-acting DNA sequences are only partially characterized. Two highly conserved motifs, thepac1andpac2motifs, lie near the ends of herpesvirus genomes and are known to be critical for genome maturation. In murine cytomegalovirus, poorly conserved sequences distal to thepac2motif up to 150 bp from the point of cleavage are also important for cleavage. Here, we sought to identify the cleavage/packaging signals of human cytomegalovirus. Our results show that a previously proposedpac2-like poly(A) tract is dispensable for cleavage/packaging function and suggest that human cytomegalovirus may utilize a crypticpac2motif that lacks a poly(A) tract characteristic ofpac2motifs in other herpesviruses. Additional distal sequences 47 to 100 bp from the point of cleavage were found to enhance cleavage efficiency. These results should facilitate the identification oftrans-acting factors that bind to theseciselements and elucidation of their functions. Such information will be critical for understanding the molecular basis of this complex process.
