8-prenylnaringenin, a novel phytoestrogen, inhibits angiogenesis in vitro and in vivo
8-prenylnaringenin, a novel phytoestrogen, inhibits angiogenesis in vitro and in vivo
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DOI：
10.1002/jcp.10460
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发表时间：
2004-04-01
影响因子：
5.6
通讯作者：
Schleuning, WD
中科院分区：
文献类型：
--
作者：
Pepper, MS;Hazel, SJ;Schleuning, WD
8-Prenylnaringenin是最近发现的植物雌激素。使用血管生成的体外模型，其中内皮细胞可以被诱导侵入三维胶原凝胶，它们在其中形成毛细血管样管，我们证明了8-异戊二烯基柚皮素抑制由碱性成纤维细胞生长因子（VGF）、血管内皮生长因子（VEGF）或两种细胞因子组合的协同作用诱导的血管生成，IC 50在3和10 μ M之间。这种效应见于来自肾上腺皮质的牛微血管内皮细胞（BME细胞）和来自牛胸主动脉的内皮细胞（BAE细胞）。发现8-prenyinaringenin的抑制作用大致等同于先前已显示在体外抑制血管生成的染料木黄酮。早期绒毛尿囊膜（CAM）测定结果显示，血管长度和静脉直径均减少，8-异戊烯基柚皮素和染料木黄酮组的效力相似。当两种物质共同添加时，观察到对CAM血管的类似作用。这些发现表明，8-异戊烯基柚皮素具有潜在的治疗应用的疾病，其中血管生成是一个重要组成部分。(C)2003 Wiley-Liss，Inc.
8-Prenylnaringenin is a recently discovered phytoestrogen. Using an in vitro model of angiogenesis in which endothelial cells can be induced to invade a three-dimensional collagen gel within which they form capillary-like tubes, we demonstrate that 8-prenylnaringenin inhibits angiogenesis induced by basic fibroblast growth factor VGF), vascular endothelial growth factor (VEGF), or the synergistic effect of the two cytokines in combination, with an IC50 of between 3 and 10 PM. This effect was seen with bovine microvascular endothelial cells derived from the adrenal cortex (BME cells) and with endothelial cells from the bovine thoracic aorta (BAE cells). The inhibitory effects of 8-prenyinaringenin were found to be roughly equipotent to those of genistein that has previously been shown to inhibit angiogenesis in vitro. Early chorioallantoic membrane (CAM) assay results showed reductions in both vessel lengths and vein diameters, with similar potency in the 8-prenylnaringenin and genistein groups. Similar effects on the CAM vessels were seen when the two substances were co-added. These findings suggest that 8-prenylnaringenin has potential therapeutic applications for diseases in which angiogenesis is an important component. (C) 2003 Wiley-Liss, Inc.