Small interfering RNA and gene silencing in transgenic mice and rats
Small interfering RNA and gene silencing in transgenic mice and rats
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DOI：
10.1016/s0014-5793(02)03680-3
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发表时间：
2002-12-04
期刊：
影响因子：
3.5
通讯作者：
Okabe, M
中科院分区：
文献类型：
--
作者：
Hasuwa, H;Kaseda, K;Okabe, M
在合成的21-23 nt RNA（siRNA）的短双链体被报道在沉默特定基因中是有效的之后，在哺乳动物培养的细胞系中证明了siRNA的基于载体的方法。然而，RNA干扰（RNAi）对活体动物中各种分化细胞的影响仍然未知。在这份报告中，我们证明，转基因提供的siRNA可以沉默无处不在表达增强绿色荧光蛋白在小鼠和大鼠身体的每一个部分。这些结果表明，转基因RNA干扰可以通过对胚胎干（ES）细胞应用同源重组来作为基因沉默的替代方法，并且即使在ES细胞系尚未建立的物种中也应该是成功的。(C)2002年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.