Acquisition of iron by Trichodesmium and associated bacteria in culture.
Acquisition of iron by Trichodesmium and associated bacteria in culture.
复制标题
培养物中的Trichodesmium 和相关细菌获取铁。
DOI：
10.1111/j.1462-2920.2011.02653.x
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发表时间：
2012
影响因子：
5.1
通讯作者：
M. Haygood
中科院分区：
文献类型：
--
作者：
Kelly L. Roe;K. Barbeau;Elizabeth L. Mann;M. Haygood
束毛藻菌落中含有丰富的微生物菌群，可能在殖民地内的营养循环中发挥作用。本研究使用Trichodesmium的实验室培养物和Trichodesmium相关微生物的两种典型细菌的基因组测序菌株，以了解Trichodesmium菌落内铁（一种潜在的限制性微量营养素）的循环。我们发现，相对于氯化铁或柠檬酸盐相关铁，铁铁载体去铁胺B和好氧菌素不容易被束毛藻生物利用。相比之下，我们研究的代表性细菌菌株能够从所有铁源中获得铁，这意味着天然存在的Trichodesmium相关细菌可能能够利用比Trichodesmium更多样化的铁源。从本研究中收集的生物体特异性吸收数据，设计了一个理论上的束毛藻菌落来模拟整个菌落的铁吸收。细菌占殖民地获得的铁的大部分（> 70%），突出了在复杂环境中确定生物体特异性吸收的重要性。 我们的研究结果表明，虽然它们可能共享相同的微环境，但束毛藻及其菌落相关的微生物群体在铁获取策略方面可能存在很大差异。
Trichodesmium colonies contain an abundant microbial consortium that is likely to play a role in nutrient cycling within the colony. This study used laboratory cultures of Trichodesmium and two genome-sequenced strains of bacteria typical of Trichodesmium-associated microbes to develop an understanding of the cycling of iron, a potentially limiting micronutrient, within Trichodesmium colonies. We found that the ferric siderophores desferrioxamine B and aerobactin were not readily bioavailable to Trichodesmium, relative to ferric chloride or citrate-associated iron. In contrast, the representative bacterial strains we studied were able to acquire iron from all of the iron sources, implying that naturally occurring Trichodesmium-associated bacteria may be capable of utilizing a more diverse array of iron sources than Trichodesmium. From the organism-specific uptake data collected in this study, a theoretical Trichodesmium colony was designed to model whole colony iron uptake. The bacteria accounted for most (> 70%) of the iron acquired by the colony, highlighting the importance of determining organism-specific uptake in a complex environment. Our findings suggest that, although they may share the same micro-environment, Trichodesmium and its colony-associated microbial cohort may differ substantially in terms of iron acquisition strategy.