Microbial Ecosystem Analysis in Root Canal Infections Refractory to Endodontic Treatment
Microbial Ecosystem Analysis in Root Canal Infections Refractory to Endodontic Treatment
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DOI：
10.1016/j.joen.2016.05.014
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发表时间：
2016-08-01
影响因子：
4.2
通讯作者：
Ribeiro Sobrinho, Antonio Paulino
中科院分区：
文献类型：
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作者：
Feitosa Henriques, Luiz Carlos;Neves de Brito, Luciana Carla;Ribeiro Sobrinho, Antonio Paulino
简介：本研究的目的是结合联合收割机多重置换扩增和棋盘DNA-DNA杂交，定性和定量评价根管治疗难治性感染中存在的微生物群。方法：本研究的对象是40例根管治疗无效的根尖周病变患者。用10号K型手锉刮或锉根管壁取样。采用多重置换扩增法对样品DNA进行扩增，并采用棋盘DNA-DNA杂交法对107个细菌分类群的水平进行了分析。根据样品中的平均比例（% DNA探针计数+/-平均值的标准误差），将分类群分为3个不同的微生物种群，如下：显性（>= 3.0%），亚显性（>1.6%-3.0%）和残留（
Introduction: The purpose of this study was to combine multiple displacement amplification and checkerboard DNA-DNA hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment. Methods: The subjects of this study were 40 patients presenting with periapical lesions refractory to endodontic treatment. Samples were taken by scraping or filing root canal walls with a #10 K-type hand file. Sample DNA was amplified by multiple displacement amplification, and the levels of 107 bacterial taxa were analyzed by checkerboard DNA DNA hybridization. The taxa were divided into 3 distinct microbial populations depending on their mean proportion in samples (% DNA probe counts +/- standard error of the mean) as follows: dominant (>= 3.0%), subdominant (>1.6%-3.0%), and residual (