Gene duplication and the evolution of ribosomal protein gene regulation in yeast.
Gene duplication and the evolution of ribosomal protein gene regulation in yeast.
复制标题
酵母中基因复制和核糖体蛋白基因调控的进化。
DOI：
10.1073/pnas.0911905107
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发表时间：
2010
影响因子：
11.1
通讯作者：
Regev,Aviv
中科院分区：
文献类型：
--
作者：
Wapinski,Ilan;Pfiffner,Jenna;French,Courtney;Socha,Amanda;Thompson,DawnAnne;Regev,Aviv
功能模块内基因的共表达可以在很大的进化距离上保守，而相关的调控机制可以实质性地分化。例如，核糖体蛋白（RP）基因在酿酒酵母中紧密共表达，但与之相关的反义和转基因因子在子囊菌中却惊人地不同。然而，关于这些变化对实际表达水平的功能影响或影响它们的选择性压力知之甚少。在这里，我们解决这个问题的背景下，RP基因表达的调控的演变，通过使用比较基因组学方法与跨物种的功能测定。我们表明，激活剂（Ifh1）和阻遏物（Crf1），控制RP基因在正常和应力条件下的调节inS.党aare来自复制和随后的专业化的一个单一的祖先蛋白。我们提供的证据表明，这种监管创新与RP基因在全基因组复制（WGD）事件中的复制相吻合，并且可能对于更严格地控制更高水平的RP转录本非常重要。我们发现，随后的损失的衍生阻遏物导致的损失的压力依赖性抑制的RP在真菌病原体光滑念珠菌。我们的比较计算和实验方法显示了基因复制如何限制和驱动调控进化，并提供了一个通用的策略，重建跨物种的基因调控的进化轨迹。
Coexpression of genes within a functional module can be conserved at great evolutionary distances, whereas the associated regulatory mechanisms can substantially diverge. For example, ribosomal protein (RP) genes are tightly coexpressed inSaccharomyces cerevisiae, but thecisandtransfactors associated with them are surprisingly diverged acrossAscomycotafungi. Little is known, however, about the functional impact of such changes on actual expression levels or about the selective pressures that affect them. Here, we address this question in the context of the evolution of the regulation of RP gene expression by using a comparative genomics approach together with cross-species functional assays. We show that an activator (Ifh1) and a repressor (Crf1) that control RP gene regulation in normal and stress conditions inS. cerevisiaeare derived from the duplication and subsequent specialization of a single ancestral protein. We provide evidence that this regulatory innovation coincides with the duplication of RP genes in a whole-genome duplication (WGD) event and may have been important for tighter control of higher levels of RP transcripts. We find that subsequent loss of the derived repressor led to the loss of a stress-dependent repression of RPs in the fungal pathogenCandida glabrata. Our comparative computational and experimental approach shows how gene duplication can constrain and drive regulatory evolution and provides a general strategy for reconstructing the evolutionary trajectory of gene regulation across species.