Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei
Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei
复制标题
过表达分泌途径的关键组成基因以增强里氏木霉中黑曲霉葡萄糖氧化酶的分泌
DOI：
10.1016/j.enzmictec.2017.07.007
复制
发表时间：
2017-11-01
影响因子：
3.4
通讯作者：
Su, Xiaoyun
中科院分区：
文献类型：
--
作者：
Wu, Yilan;Sun, Xianhua;Su, Xiaoyun
Vast interest exists in developing T. reesei for production of heterologous proteins. Although rich genomic and transcriptomic information has been uncovered for the T. reesei secretion pathway, little is known about whether engineering its key components could enhance expression of a heterologous gene. In this study, snc1, a v-SNARE gene, was first selected for overexpression in T. reesei. In engineered T. reesei with additional copies of snc1, the Aspergillus niger glucose oxidase (AnGOD) was produced to a significantly higher level(2.2-fold of the parental strain). hac1 and bip1, two more component genes in the secretion pathway, were further tested for over expression and found to be also beneficial for AnGOD secretion. The overexpression of one component gene more or less affected the expression of the other two genes, suggesting a complex regulating mechanism. Our study demonstrates the potential of engineering the secretion pathway for enhancing heterologous gene production in T. reesei.
