Comparison of second- and third-generation anti-cyclic citrullinated peptide antibodies assays for detecting rheumatoid arthritis
Comparison of second- and third-generation anti-cyclic citrullinated peptide antibodies assays for detecting rheumatoid arthritis
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DOI：
10.1016/j.cca.2007.08.002
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发表时间：
2007-11-01
影响因子：
5
通讯作者：
Chapelle, Jean-Paul
中科院分区：
文献类型：
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作者：
Lutteri, Laurence;Malaise, Michel;Chapelle, Jean-Paul
背景：本研究的目的是比较7种市售的第二代抗环瓜氨酸肽（CCP2）抗体、抗角蛋白抗体（AKA）和类风湿因子（RF）检测在类风湿关节炎（RA）患者中的诊断和临床价值，并检查第三代抗ccp （CCP3）抗体检测的潜在优势。方法：采用120例RA患者和170例对照者的血清标本，测定CCP2和CCP3抗体的敏感性、特异性、阳性预测值（PPV）和阴性预测值（NPV），以及AKA和RF测定。采用受试者工作特征（ROC）曲线方法对这些试验的各自性能进行比较。结果：我们发现抗ccp检测方法在敏感性和特异性上没有显著差异。我们评估的CCP3抗体检测结果并不比第二代检测结果更敏感。与RIF技术相比，所有抗ccp检测方法均具有更好的特异性，但灵敏度较低。相比之下，虽然具有相同的特异性，但它们在区分RA与其他风湿病方面比AKA更敏感。结论：抗ccp抗体检测是一种有效的诊断工具，特别是在AKA或RE阴性的RA患者中，CCP3抗体检测的诊断性能并不比第二代检测更好。(C) 2007 Elsevier B.V.版权所有
Background: The aims of the present study were to compare the diagnostic and clinical value of seven commercially available assays for second-generation anti-cyclic citrullinated peptide (CCP2) antibodies, anti-keratin antibodies (AKA) and rheumatoid factor (RF) determination in patients with rheumatoid arthritis (RA), and to check the potential advantages of a third-generation anti-CCP (CCP3) antibodies assay.Methods: Serum samples from 120 RA patients and from 170 controls were used to determine the sensitivity, the specificity, the positive (PPV) and negative predictive values (NPV) of CCP2 and CCP3 antibodies, AKA and RF assays. The respective performances of these tests were compared using a receiver-operating characteristics (ROC) curves methodology.Results: We found no significant differences in sensitivity and specificity between the tested anti-CCP assays. The CCP3 antibodies assay we evaluated was not significantly more sensitive than those of the second generation. Compared with RIF technique, all anti-CCP assays showed better specificity, but lower sensitivity. In contrast, while of equivalent specificity, they proved to be more sensitive than AKA in the discrimination of RA from other rheumatic diseases.Conclusions: Anti-CCP antibodies determination proved to be a powerful diagnostic tool, especially in RA patients with negative AKA or RE The investigated CCP3 antibodies assay had no better diagnostic performances than the second-generation assays. (C) 2007 Elsevier B.V. All rights reserved.