Identification of mRNAs regulated by interferon-gamma in cultured rat astrocytes by PCR differential display.
Identification of mRNAs regulated by interferon-gamma in cultured rat astrocytes by PCR differential display.
复制标题
通过 PCR 差异显示鉴定培养的大鼠星形胶质细胞中受干扰素-γ 调节的 mRNA。
DOI：
10.1159/000097214
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发表时间：
1995
影响因子：
2.4
通讯作者：
G. Jonakait
中科院分区：
文献类型：
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作者：
W. Kuchinke;R. Hart;G. Jonakait
我们已经使用RNA差异显示来鉴定受干扰素-γ(干扰素-伽马)处理的富集型星形胶质细胞培养物中特定的mRNA种类。在干扰素处理24小时后，使用15种不同的随机引物进行差异显示。选择105个上调和5个下调的cDNA进行进一步测序和鉴定。Northern印迹分析证实有13个基因表达上调，这些基因与以下基因相同或高度相似：分泌间肾上腺髓质素、H-rev 107、CAP样蛋白、三磷酸腺苷合成酶epsilon亚基、补体C3、S-100β、钙离子ATPase、mg11、干扰素上调的56-kD蛋白、层粘连蛋白受体样蛋白、蛋白酪氨酸磷酸酶和zic。这些数据表明，暴露于干扰素-γ会导致星形胶质细胞基因表达模式的复杂变化。
We have used RNA differential display to identify specific mRNA species that are regulated by interferon-gamma (IFN gamma) treatment of enriched astrocyte cultures. Following a 24-hour treatment with IFN, mRNA was subjected to differential display using 15 different random primers. 105 upregulated and 5 downregulated cDNAs were selected for further sequencing and identification. Northern blot analysis confirmed the upregulation of 13 genes identical or highly similar to: intercrine adrenomedullin, H-rev 107, CAP-like protein, ATP synthase epsilon-subunit, complement C3, S-100 beta, Ca2+ ATPase, mg11, IFN-upregulated 56-kD protein mRNA, laminin receptor-like protein, protein tyrosine phosphatase, and zic. These data suggest that exposure to IFN gamma results in a complex change in the pattern of astrocyte gene expression.