Fluorimetric assay of mycobacterial group-specific hydrolase enzymes.
Fluorimetric assay of mycobacterial group-specific hydrolase enzymes.
复制标题
分枝杆菌组特异性水解酶的荧光测定。
DOI：
10.1136/jcp.31.4.378
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发表时间：
1978
影响因子：
3.4
通讯作者：
J. Grange
中科院分区：
文献类型：
--
作者：
J. Grange
由4-甲基伞形酮制备的生氟底物为检测和分析少量完整分枝杆菌的组特异性水解酶活性提供了一种简单、方便的方法。还可以研究酶的物理化学性质，如pH依赖性和热稳定性。本文介绍了一种研究七种分枝杆菌的糖苷酶、氨基己糖苷酶、酸性磷酸酶和酰基酯酶活性的技术。这种技术将有助于分枝杆菌的分类、鉴定和量化，特别是生长缓慢的菌株，其中的生化特性很难用其他技术检测出来。
Fluorigenic substrates prepared from 4-methylumbelliferone provide a simple, convenient method for detecting and assaying group-specific hydrolase activity in small quantities of whole mycobacteria. Physico-chemical properties of the enzymes such as pH dependence and heat stability may also be studied. A technique is described for studying glycosidase, hexosaminidase, acid phosphatase, and acyl esterase activity in seven mycobacterial species. Such techniques will be a useful aid to the taxonomy, identification, and quantification of mycobacteria, especially slowly growing strains in which biochemical properties are very difficult to detect by other techniques.