Characterization of the cDNA coding for mouse prothrombin and localization of the gene on mouse chromosome 2.
Characterization of the cDNA coding for mouse prothrombin and localization of the gene on mouse chromosome 2.
复制标题
编码小鼠凝血酶原的 cDNA 的表征以及该基因在小鼠 2 号染色体上的定位。
DOI：
10.1089/dna.1990.9.487
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发表时间：
1990
影响因子：
3.1
通讯作者：
Elliott,RW
中科院分区：
文献类型：
--
作者：
Degen,SJ;Schaefer,LA;Jamison,CS;Grant,SG;Fitzgibbon,JJ;Pai,JA;Chapman,VM;Elliott,RW
已分离到一系列编码小鼠凝血酶原(凝血因子II)的重叠cDNA，并确定了其复合DNA序列。完整的凝血酶原基因全长1,987个碱基对，编码18个碱基的5‘非翻译序列、一个编码618个氨基酸的开放阅读框、一个终止密码子和一个112个碱基的3’非翻译区和一个多聚(A)尾巴。翻译的氨基酸序列预测其相对分子质量为66,087，其中包括10个γ-羧基谷氨酸残基。有五个潜在的N-连接糖基化位点。小鼠凝血酶原蛋白与人和牛的同源性分别为81.4%和77.3%。小鼠凝血酶原基因与人和牛的同源性分别为79.9%和76.5%。对人类凝血酶原的结构和功能至关重要的氨基酸残基在小鼠和牛的蛋白质中是保守的。在成年小鼠和大鼠中，凝血酶原主要在肝脏合成，经溶液杂交分析，凝血酶原占总mRNA的0.07%。利用种间回交和两个物种之间的DNA片段差异，已经定位了小鼠凝血酶原基因Cf-2的遗传位点。凝血酶原基因位于小鼠2号染色体上，过氧化氢酶基因座近端1.8±1.3个MAP单位。该区域的基因序列为Cen-Acra-Cf-2-Cas-1-A-Tel。这一定位将已知的小鼠2号染色体与人11p染色体同源区域的近端边界从Cas-1约2个图谱单位延伸到着丝粒。
A series of overlapping cDNAs coding for mouse prothrombin (coagulation factor II) have been isolated and the composite DNA sequence has been determined. The complete prothrombin cDNA is 1,987 bp in length [excluding the poly(A) tail] and codes for 18 bp of 5′ untranslated sequence, an open reading frame coding for 618 amino acids, a stop codon, and a 3′ untranslated region of 112 bp followed by a poly(A) tail. The translated amino acid sequence predicts a molecular weight of 66,087, which includes 10 residues of γ-carboxyglutamic acid. There are five potentialN-linked glycosylation sites. Mouse prothrombin is 81.4% and 77.3% identical to the human and bovine proteins, respectively. Comparison of the cDNA coding for mouse prothrombin to the human and bovine cDNAs indicates 79.9% and 76.5% identity, respectively. Amino acid residues important for the structure and function of human prothrombin are conserved in the mouse and bovine proteins. In the adult mouse and rat, prothrombin is primarily synthesized in the liver, where is constitutes 0.07% of total mRNA as determined by solution hybridization analysis. The genetic locus for mouse prothrombin,Cf-2, has been mapped using an interspecies backcross and DNA fragment differences between the two species. The prothrombin locus lies on mouse chromosome 2, 1.8 ± 1.3 map units proximal to the catalase locus. The gene order in this region isCen-Acra-Cf-2-Cas-1-A-Tel. This localization extends the proximal boundary of the known region of homology between mouse chromosome 2 and human chromosome 11p fromCas-1about 2 map units toward the centromere.