Mouse neutrophils are professional antigen-presenting cells programmed to instruct Th1 and Th17 T-cell differentiation
Mouse neutrophils are professional antigen-presenting cells programmed to instruct Th1 and Th17 T-cell differentiation
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DOI：
10.1093/intimm/dxr007
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发表时间：
2011-05-01
影响因子：
4.4
通讯作者：
Denkers, Eric Y.
中科院分区：
文献类型：
--
作者：
Abdallah, Delbert S. Abi;Egan, Charlotte E.;Denkers, Eric Y.
中性粒细胞在先天免疫系统中起主要作用，通常被认为是发挥抗微生物活性的短命效应细胞，有时还具有免疫病理学作用。在这里，我们证明这些细胞具有作为专业抗原提呈细胞的额外功能，能够启动T(H)1和T(H)17获得性免疫反应。通过流式细胞仪、荧光显微镜和免疫印迹等方法，我们发现T细胞共孵育后，小鼠中性粒细胞表达MHC-II类分子和共刺激分子CD80、CD86。中性粒细胞以卵清蛋白(OVA)过程冲击，以MHC II类依赖的方式向OVA特异性T细胞递呈多肽抗原。重要的是，我们证明中性粒细胞可以启动抗原特异性T(H)1和T(H)17免疫反应，即使在细胞培养中不添加外源细胞因子。
Neutrophils play a major role in the innate immune system and are normally considered to be short-lived effector cells that exert anti-microbial activity and sometimes immunopathology. Here, we show that these cells possess an additional function as professional antigen-presenting cells capable of priming a T(h)1- and T(h)17-acquired immune response. Using flow cytometry, fluorescence microscopy and western blotting, we show that mouse neutrophils express MHC class II and co-stimulatory molecules CD80 and CD86 after T-cell co-incubation. Neutrophils pulsed with ovalbumin (OVA) process and present peptide antigen to OVA-specific T cells in an MHC class II-dependent manner. Importantly, we demonstrate that neutrophils can prime antigen-specific T(h)1 and T(h)17 immune responses even without the addition of exogenous cytokines to cell cultures.