Macroporous silicon chips for laterally resolved, multi-parametric analysis of epithelial barrier function.
Macroporous silicon chips for laterally resolved, multi-parametric analysis of epithelial barrier function.
复制标题
用于上皮屏障功能横向解析多参数分析的大孔硅芯片。
DOI：
10.1039/c2lc00026a
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发表时间：
2012
期刊：
影响因子：
6.1
通讯作者：
J. Wegener
中科院分区：
文献类型：
--
作者：
S. Michaelis;Christina E. Rommel;J. Endell;P. Göring;R. Wehrspohn;C. Steinem;A. Janshoff;H. Franke;J. Wegener
这项研究描述了一种新的方法，可以用亚细胞的横向分辨率来可视化上皮细胞层和内皮细胞层的大分子渗透性。揭示了细胞层中的缺陷和有关迁移溶质渗透路径的细节。该分析是基于硅片，芯片的一侧是密集排列的、高度有序的、死胡同的μm直径的小孔。研究中的细胞生长在芯片的多孔侧，使生长表面的孔作为一系列毫微升大小的试管，其中渗透探针在渗透部位聚集。孔隙填充模式揭示了细胞层的渗透性特征，横向分辨率在μm范围内。芯片表面涂有一层薄薄的金层，可以对附着的细胞进行阻抗分析，以便同时测量它们对无机离子的紧密性。这项新的检测提供了一种前所未有的关于上皮和内皮屏障功能的观察。
This study describes a novel assay to visualize the macromolecular permeability of epithelial and endothelial cell layers with subcellular lateral resolution. Defects within the cell layer and details about the permeation route of the migrating solute are revealed. The assay is based on silicon chips with densely packed, highly ordered, dead-ended pores of μm-diameters on one side. The cells under study are grown on the porous side of the chip such that the pores in the growth surface serve as an array of femtolitre-sized cuvettes in which the permeating probe accumulates at the site of permeation. The pattern of pore filling reveals the permeability characteristics of the cell layer with a lateral resolution in the μm range. Coating of the chip surface with a thin layer of gold allows for impedance analysis of the adherent cells in order to measure their tightness for inorganic ions at the same time. The new assay provides an unprecedented look on epithelial and endothelial barrier function.