Purification of growth hormone-specific transcription factor GHF-1 containing homeobox.
Purification of growth hormone-specific transcription factor GHF-1 containing homeobox.
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含有同源盒的生长激素特异性转录因子 GHF-1 的纯化。
DOI：
10.1126/science.2563596
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Karin,M
中科院分区：
文献类型：
--
作者：
Castrillo,JL;Bodner,M;Karin,M
生长激素 (GH) 基因的垂体特异性表达由转录因子 GHF-1 控制，该转录因子与其启动子内的两个位点结合。最近，GHF-1 被证明是 DNA 结合蛋白同源框家族的成员。一个重要的问题是 GHF-1 是否控制其他垂体特定基因的表达，例如在密切相关的细胞类型中表达的催乳素 (Prl)。为此，从表达 GH 和 Prl 的垂体肿瘤细胞提取物中纯化 GHF-1，并鉴定为 33 千道尔顿的多肽。尽管GHF-1结合并激活GH启动子，但它不识别Prl启动子。然而，同一提取物中至少有一个其他因子（很容易与 GHF-1 分离）与 Pr1 内的多个位点结合，但不与 GH 启动子结合。 GHF-1 抗体不与 Prl 结合活性发生反应。这些结果意味着 GH 和 Prl 的垂体特异性表达受两种不同的反式作用因子控制。
Pituitary-specific expression of the growth hormone (GH) gene is governed by a transcription factor, GHF-1, that binds to two sites within its promoter. Recently, GHF-1 was shown to be a member of the homeobox family of DNA-binding proteins. An important question is whether GHF-1 controls the expression of other pituitary specific genes, such as prolactin (Prl), expressed in closely related cell types. To this end, GHF-1 was purified from extracts of GH- and Prl-expressing pituitary tumor cells and identified as a 33-kilodalton polypeptide. Although GHF-1 bound to and activated the GH promoter, it did not recognize the Prl promoter. However, at least one other factor in the same extracts, which was easily separated from GHF-1, bound to several sites within the Prl but not the GH promoter. Antibodies to GHF-1 did not react with the Prl binding activity. These results imply that the pituitary-specific expression of GH and Prl is governed by two distinct trans-acting factors.