Inactivation of viral and prion pathogens by γ-irradiation under conditions that maintain the integrity of human albumin
Inactivation of viral and prion pathogens by γ-irradiation under conditions that maintain the integrity of human albumin
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DOI：
10.1046/j.1423-0410.2003.00256.x
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发表时间：
2003-01-01
期刊：
影响因子：
2.7
通讯作者：
Drohan, WN
中科院分区：
文献类型：
--
作者：
Miekka, SI;Forng, RY;Drohan, WN
背景和目的治疗性血浆蛋白浓缩物的给药与传播病毒疾病的真实风险和Pron传播的理论风险相关。材料与方法人白蛋白25%溶液，加入4种模型病毒(包括猪细小病毒)或从感染瘙痒病的仓鼠脑匀浆中，以恒定的低剂量率进行伽马照射，检测病毒和普恩病毒的感染性或白蛋白完整性。结果在50kGY的辐射剂量下，病毒灭活大于或等于3.2，大于或等于6.4log(10)，瘙痒病估计为1.5log(10)，而白蛋白仅呈中度聚集和碎裂。结论伽玛射线照射可优先灭活病毒和普恩病原体，且不会对白蛋白结构造成过大破坏。
Background and Objectives The administration of therapeutic plasma protein concentrates has been associated with the real risk of transmitting viral diseases and the theoretical risks of prion transmission. Our objective was to determine if gamma-irradiation can inactivate viral or prion infectivity without damaging a protein biotherapeutically.Materials and Methods Human albumin 25% solution, spiked with four model viruses (including porcine parvovirus) or with brain homogenate from scrapie-infected hamsters, was gamma-irradiated at constant low-dose rates and assayed for viral and prion infectivity or for albumin integrity.Results At a radiation dose of 50 kGy, viruses were inactivated by greater than or equal to 3.2 to greater than or equal to 6.4 log(10) and scrapie by an estimated 1.5 log(10), whereas albumin was only moderately aggregated and fragmented.Conclusions gamma-Irradiation can preferentially inactivate viral and prion pathogens without excessive damage to albumin structure.