Functional heterogeneity of memory B lymphocytes: in vivo analysis of TD-primed B cells responsive to secondary stimulation with TD and TI antigens.
Functional heterogeneity of memory B lymphocytes: in vivo analysis of TD-primed B cells responsive to secondary stimulation with TD and TI antigens.
复制标题
记忆 B 淋巴细胞的功能异质性：体内分析 TD 引发的 B 细胞对 TD 和 TI 抗原二次刺激的反应。
DOI：
10.4049/jimmunol.131.2.561
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发表时间：
1983
影响因子：
4.4
通讯作者：
E. Benjamini
中科院分区：
文献类型：
--
作者：
D. Rennick;P. R. Morrow;E. Benjamini
分析了由烟草花叶病毒蛋白(TMVP)103-112氨基酸残基组成的单一决定簇诱导的记忆B细胞的功能异质性。过继转移TMVP免疫脾细胞的小鼠在TMVP、与琥珀酸化人丙种球蛋白(SHGG)结合的十肽或与流产布鲁氏菌(BA)结合的十肽攻击时，可产生十肽特异性抗体。而TMVP或十肽-SHGG的二次刺激依赖于适当的T细胞刺激，而十肽-BA的刺激不依赖于常规的T细胞帮助。此外，记忆B细胞对TMVP(Td)、十肽-SHGG(Td)或十肽-BA(TI.TMVP诱导的细胞与TD和TI十肽抗原同时攻击的过继受体不会产生比单独注射其中一种TD抗原更高的抗体应答。然而，十肽-BA始终比TMVP或十肽-SHGG诱导更小的反十肽反应。这表明，只有一小部分被原始启动抗原(胸腺依赖)激活的记忆B细胞群对与布鲁氏菌结合的启动半抗原在体内的二次刺激也有反应。在TMVP、十肽-SHGG或十肽-BA二次攻击后，对TMVP免疫脾细胞诱导的抗体的详细分析未能区分反应性记忆B细胞；三种免疫原诱导的抗十肽抗体具有相同的精细特异性和免疫球蛋白同型组成。这些数据被认为是进一步的证据，表明TD免疫的B细胞亚群可能对TD和TI形式的抗原交叉刺激表现出不同的敏感性，代表着共同B细胞谱系中记忆B细胞成熟的不同阶段。为了支持这一结论，我们在接下来的交流中建立了TI和/或TD反应性十肽记忆B细胞之间的发育关系。
The functional heterogeneity of memory B cells induced by a single determinant, consisting of a decapeptide representing amino acid residues 103-112 of tobacco mosaic virus protein (TMVP), was analyzed. Decapeptide specific antibodies were elicited in mice adoptively transferred with TMVP-immune spleen cells when challenged with TMVP, decapeptide conjugated to succinylated human gamma-globulin (SHGG), or decapeptide conjugated to Brucella abortus (BA). Whereas secondary stimulation by either TMVP or decapeptide-SHGG was dependent on appropriately primed T cells, stimulation by decapeptide-BA was independent of conventional T cell help. Furthermore, memory B cells responsive to TMVP (TD), decapeptide-SHGG (TD), or decapeptide-BA (TI. 1 prototype) were shown to consist of overlapping populations because adoptive recipients of TMVP-primed cells challenged simultaneously with TD and TI decapeptide antigens did not result in a higher antibody response than that elicited by one of the TD antigens injected alone. However, decapeptide-BA consistently induced a smaller antidecapeptide response than either TMVP or decapeptide-SHGG. This suggested that only a fraction of the memory B cell population which was activated by the original priming antigen (thymus-dependent) was also responsive to secondary in vivo stimulation by the priming hapten conjugated to Brucella abortus. Detailed analyses of the antibodies induced in the recipients of TMVP-immune spleen cells after secondary challenge with either TMVP, decapeptide-SHGG, or decapeptide-BA failed to distinguish between the responsive memory B cells; the antidecapeptide antibodies induced by all three immunogens shared the same fine specificities and immunoglobulin isotype composition. These data are viewed as further evidence that subsets of TD-primed B cells, which may display differential sensitivity to cross-stimulation with TD and TI forms of the antigen, represent distinct stages of memory B cell maturation within a common B cell lineage. In support of this conclusion, we establish a developmental relationship between TI and/or TD responsive decapeptide memory B cell in the following communication.