Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems
Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems
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DOI：
10.1073/pnas.0610868104
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发表时间：
2007-11-27
影响因子：
11.1
通讯作者：
Takayama, Shuichi
中科院分区：
文献类型：
--
作者：
Huh, Dongeun;Fujioka, Hideki;Takayama, Shuichi
我们描述了一种集成了计算机化气液两相微流体的微制造气道系统，该系统能够在芯片上对人类气道上皮进行工程设计，并精确复制呼吸系统中发现的生理性或病理性液体塞流。使用该装置，我们展示了在血流条件下细胞水平的肺损伤，引起多种肺部疾病的症状特征。具体来说，模拟缺乏表面活性剂的封闭气道重新开放的液体塞的扩展和破裂会产生有害的流体机械应力，从而导致气道小上皮细胞的严重损伤。我们还表明，由塞子破裂产生的爆炸压力波能够以爆裂声的形式检测机械细胞损伤。
We describe a microfabricated airway system integrated with computerized air-liquid two-phase microfluiclics that enables on-chip engineering of human airway epithelia and precise reproduction of physiologic or pathologic liquid plug flows found in the respiratory system. Using this device, we demonstrate cellular-level lung injury under flow conditions that cause symptoms characteristic of a wide range of pulmonary diseases. Specifically, propagation and rupture of liquid plugs that simulate surfactant-deficient reopening of closed airways lead to significant injury of small airway epithelial cells by generating deleterious fluid mechanical stresses. We also show that the explosive pressure waves produced by plug rupture enable detection of the mechanical cellular injury as crackling sounds.