Clonal Sequences Recovered from Plasma from Patients with Residual HIV-1 Viremia and on Intensified Antiretroviral Therapy Are Identical to Replicating Viral RNAs Recovered from Circulating Resting CD4+ T Cells
Clonal Sequences Recovered from Plasma from Patients with Residual HIV-1 Viremia and on Intensified Antiretroviral Therapy Are Identical to Replicating Viral RNAs Recovered from Circulating Resting CD4+ T Cells
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DOI：
10.1128/jvi.00284-11
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发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
Margolis, David M.
中科院分区：
文献类型：
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作者：
Anderson, Jeffrey A.;Archin, Nancie M.;Margolis, David M.
尽管成功的抗逆转录病毒治疗（ART），低水平的病毒血症（LLV）可能会间歇性地检测到大多数HIV感染患者。从两名接受抑制性ART的患者中获得纵向血浆和静息CD 4（+）T细胞，以研究LLV的来源。对来自LLV血浆的HIV-1 env进行单基因组测序，并将序列与从静息CD 4（+）T细胞的有限稀释生长试验中回收的序列进行比较。循环的LLV病毒克隆与从数百万静息CD 4（+）T细胞库的生长测定中回收的病毒相同。了解LLV的来源需要评估所有可能的HIV持续感染的储存库。
Despite successful antiretroviral therapy (ART), low-level viremia (LLV) may be intermittently detected in most HIV-infected patients. Longitudinal blood plasma and resting CD4(+) T cells were obtained from two patients on suppressive ART to investigate the source of LLV. Single-genome sequencing of HIV-1 env from LLV plasma was performed, and the sequences were compared to sequences recovered from limiting-dilution outgrowth assays of resting CD4(+) T cells. The circulating LLV virus clone was identical to virus recovered from outgrowth assays from pools of millions of resting CD4(+) T cells. Understanding the sources of LLV requires evaluation of all possible reservoirs of persistent HIV infection.