Musculotopic organization of the motor neurons supplying forelimb and shoulder girdle muscles in the mouse
Musculotopic organization of the motor neurons supplying forelimb and shoulder girdle muscles in the mouse
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DOI：
10.1007/s00429-012-0396-3
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发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Watson, Charles
中科院分区：
文献类型：
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作者：
Bacskai, Timea;Fu, Yuhong;Watson, Charles
我们使用氟金逆行示踪剂注射确定了 C57BL/6J 小鼠脊髓中供应肩带和前肢肌肉的运动神经元 (MN)。在从 C2 到 T2 的脊髓横断面中，我们观察到两个 MN 柱（内侧和外侧），均具有腹侧和背侧细分。背外侧柱由肱二头肌、前臂伸肌、前臂屈肌和手 MN 组成，腹外侧柱由背阔肌、斜方肌、大圆肌、三角肌和三头肌 MN 组成。冈上肌 MN 位于背内侧柱，胸大肌和前锯肌 MN 位于腹内侧柱。背外侧柱的 MN 支配 C4 中部至 C7 中部的肱二头肌、C4 尾部至 T1 中部的前臂伸肌、C5 喙部至 T1 中部的前臂屈肌以及 C8 中部至 T2 中部的手部肌肉。支配斜方肌的 MN 位于 C2 中部至 C4 中部，肱三头肌位于 C6 中部至 T1 头端，三角肌位于 C4 头端至 C6 中部，大圆肌位于 C5 头端至 C8 中部，背阔肌位于 C5 中部至 C8 尾部。此外，支配冈上肌的 MN 位于从 C4 头端到 C8 尾端，C6 中部到 T2 中部的胸大肌，以及 C5 头端到 C7/C8 尾端的前锯肌。虽然 MN 群体的肌肉分布模式与其他物种记录的非常相似，但我们发现一些 MN 池的位置和头尾范围与之前的报告相比存在差异。小鼠前肢 MN 的鉴定可以作为研究退行性 MN 疾病、脊髓损伤和发育基因表达的解剖学参考。
We identified the motor neurons (MNs) supplying the shoulder girdle and forelimb muscles in the C57BL/6J mouse spinal cord using Fluoro-Gold retrograde tracer injections. In spinal cord transverse sections from C2 to T2, we observed two MN columns (medial and lateral) both with ventral and dorsal subdivisions. The dorsolateral column consisted of the biceps brachii, forearm extensors, forearm flexors, and hand MNs, and the ventrolateral column consisted of the latissimus dorsi, trapezius, teres major, deltoid, and triceps MNs. The supraspinatus muscle MNs were located in the dorsomedial column, and pectoralis major and serratus anterior MNs were located in the ventromedial columns. MNs of the dorsolateral column innervated the biceps brachii in mid-C4 to mid-C7, forearm extensors in caudal C4 to mid-T1, forearm flexors in rostral C5 to mid-T1, and hand muscles in mid-C8 to mid-T2 segments. The MNs innervating the trapezius were located in mid-C2 to mid-C4, triceps brachii in mid-C6 to rostral T1, deltoid in rostral C4 to mid-C6, teres major in rostral C5 to mid-C8, and latissimus dorsi in mid-C5 to caudal C8. In addition, MNs innervating the supraspinatus were located from rostral C4 to caudal C8, pectoralis major in mid-C6 to mid-T2, and serratus anterior in rostral C5 to caudal C7/rostral C8 segments. While the musculotopic pattern of MN groups was very similar to that documented for other species, we found differences in the position and cranio-caudal extent of some MN pools compared with previous reports. The identification of mouse forelimb MNs can serve as an anatomical reference for studying degenerative MN diseases, spinal cord injury, and developmental gene expression.