Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy
Cartilage formation in a hollow fiber bioreactor studied by proton magnetic resonance microscopy
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DOI：
10.1016/s0945-053x(98)90099-3
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发表时间：
1998-11-01
期刊：
影响因子：
6.9
通讯作者：
Spencer, RGS
中科院分区：
文献类型：
--
作者：
Potter, K;Butler, JJ;Spencer, RGS
用于研究软骨形成和维持调节的理想体外系统将允许三维组织生长、广泛的生化干预和无损评估。我们开发了符合这些标准的中空纤维生物反应器（HFBR）系统。注射胚胎鸡胸骨软骨细胞后，中空纤维周围会形成新软骨，生长四个星期后厚度可达一毫米。随着时间的推移，通过核磁共振（NMR）显微成像以及相关的生化和组织学分析来监测这一过程。在发育过程中，组织体积和细胞构成大大增加。这伴随着与大分子含量增加一致的磁共振特性的变化。此外，在定量 NMR 图像中也观察到了组织异质性，即组织切片中细胞大小的区域变化。
The ideal in vitro system for investigating the regulation of cartilage formation and maintenance would allow for three-dimensional tissue growth, a wide range of biochemical interventions, and non-destructive evaluation. We have developed a hollow fiber bioreactor (HFBR) system which meets these criteria. After injection with embryonic chick sternal chondrocytes, neocartilage is elaborated around the hollow fibers, reaching a thickness of up to a millimeter after four weeks of growth. This process was monitored over time with nuclear magnetic resonance (NMR) microimaging and correlative biochemical and histologic analyses. Tissue volume and cellularity increased greatly during development. This was accompanied by changes in magnetic resonance properties consistent with increased macromolecular content. Further, tissue heterogeneity, observed as regional variations in cell size in histologic sections, was also observed in quantitative NMR images.