Bioaccumulation and toxicity of CdSe/ZnS quantum dots in Phanerochaete chrysosporium.
Bioaccumulation and toxicity of CdSe/ZnS quantum dots in Phanerochaete chrysosporium.
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DOI：
10.1016/j.colsurfb.2017.08.006
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发表时间：
2017-11
期刊：
影响因子：
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通讯作者：
L. Hu;G. Zeng;Gui-qiu Chen;Zhenzhen Huang;Jia Wan;Anwei Chen;Zhigang Yu;Jiangliu Yang;Kai He
中科院分区：
文献类型：
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作者：
L. Hu;G. Zeng;Gui-qiu Chen;Zhenzhen Huang;Jia Wan;Anwei Chen;Zhigang Yu;Jiangliu Yang;Kai He
量子点在生物医学应用中的潜力越来越大，这引起了人们对其毒理学影响的相当大的关注。因此，有必要了解量子点潜在的毒性机制。在这项工作中，我们利用激光共聚焦扫描显微镜、活性氧(ROS)测定和细胞活力测定等方法，全面研究了三种CdSe/ZnS量子点(COOH、CdSe/ZnS 525、NH_2CdSe/ZnS_(625))在黄孢原毛平革菌(P.chrysosporium)中的生物积累和毒性。激光共聚焦扫描显微镜分析结果表明，浓度在10~80nM范围内的CdSe/ZnS量子点均能在菌丝中大量积累并诱导ROS的产生，表现出直接的毒性峰。黄孢子菌。CdSe/ZnS量子点的生物蓄积和毒性对NP具有剂量依赖和时间依赖的效应。黄孢子菌。此外，CdSe/ZnS量子点的细胞毒性也与其物理化学性质有关，包括颗粒大小和表面电荷：与大尺寸的NH2 CdSe/ZnS 625相比，小尺寸的NH_2CdSe/ZnS 525具有更强的细胞毒性，而较小的负电荷的NH_2CDSe/ZnS_525的细胞毒性大于较大的负电荷的COOH_cDe/ZnS_525。所得结果为探索和理解量子点在活细胞中的毒性机制提供了有价值的信息。
The growing potential of quantum dots (QDs) in biomedical applications has raised considerable concerns regarding their toxicological impact. Consequently, there has been a need to understand the underlying toxicity mechanism of QDs. In this work, we comprehensively investigated the bioaccumulation and toxicity of three CdSe/ZnS QDs (COOH CdSe/ZnS 525, NH2CdSe/ZnS 525, and NH2CdSe/ZnS 625) inPhanerochaete chrysosporium(P. chrysosporium) using confocal laser scanning microscopy, reactive oxygen species (ROS) measurements, and cell viability assays. Confocal laser scanning microscopy analytical results indicated that all the CdSe/ZnS QDs, with the concentration ranging from 10 to 80 nM, could accumulate largely in the hyphae and induce the generation of ROS, showing a direct toxicity toP. chrysosporium. And the bioaccumulation and toxicity of CdSe/ZnS QDs presented dose-dependent and time-dependent effects onP. chrysosporium. Furthermore, the CdSe/ZnS QDs-induced cytotoxicity was also related to their physicochemical properties, including particle size and surface charges: NH2CdSe/ZnS 525 with small size was more cytotoxic as compared to NH2CdSe/ZnS 625 with large size, and the smaller negative charged NH2CdSe/ZnS 525 resulted in greater cytotoxicity than the larger negative charged COOH CdSe/ZnS 525. The obtained results provide valuable information for exploring and understanding of toxicity mechanism of QDs in living cells.