AN UNMODIFIED HEPTADECAPEPTIDE PHEROMONE INDUCES COMPETENCE FOR GENETIC-TRANSFORMATION IN STREPTOCOCCUS-PNEUMONIAE
AN UNMODIFIED HEPTADECAPEPTIDE PHEROMONE INDUCES COMPETENCE FOR GENETIC-TRANSFORMATION IN STREPTOCOCCUS-PNEUMONIAE
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DOI：
10.1073/pnas.92.24.11140
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发表时间：
1995-11-21
影响因子：
11.1
通讯作者：
MORRISON, DA
中科院分区：
文献类型：
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作者：
HAVARSTEIN, LS;COOMARASWAMY, G;MORRISON, DA
三十年来，人们一直知道肺炎链球菌的遗传转化能力是在临界细胞密度的生长培养物中产生的，以响应分泌的蛋白酶敏感信号。我们表明，菌株CP 1200产生一个17个残基的肽，诱导该物种的细胞发展的能力。其序列为H-Glu-Met-Arg-Leu-Ser-Lys-Phe-Phe-Arg-Asp-Phe-Ile-Leu-Gln-Arg-Lys-Lys-OH。相同序列的合成肽显示出少量的生物活性，并扩展了适合于感受态形成的条件范围。肺炎球菌染色体中的同源密码子表明这种肽是由核糖体合成的。由于该基因编码含有在肽细菌素中发现的Gly-Gly共有加工位点的前肽，因此该肽很可能通过专门的ATP结合盒转运蛋白输出，这是这些细菌素的特征。提出的假设是，这种转运蛋白编码的comA，以前被证明是需要制定的肺炎球菌的能力激活剂。
Competence for genetic transformation in Streptococcus pneumoniae has been known for three decades to arise in growing cultures at a critical cell density, in response to a secreted protease-sensitive signal. We show that strain CP1200 produces a 17-residue peptide that induces cells of the species to develop competence. The sequence of the peptide was found to be H-Glu-Met-Arg-Leu-Ser-Lys-Phe-Phe-Arg-Asp-Phe-Ile-Leu-Gln-Arg-Lys-Lys-OH. A synthetic peptide of the same sequence was shown to be biologically active in small quantities and to extend the range of conditions suitable for development of competence. Cognate codons in the pneumococcal chromosome indicate that the peptide is made ribosomally. As the gene encodes a prepeptide containing the Gly-Gly consensus processing site found in peptide bacteriocins, the peptide is likely to be exported by a specialized ATP-binding cassette transport protein as is characteristic of these bacteriocins. The hypothesis is presented that this transport protein is encoded by comA, previously shown to be required for elaboration of the pneumococcal competence activator.