Green tea protects against hippocampal neuronal apoptosis in diabetic encephalopathy by inhibiting JNK/MLCK signaling.
Green tea protects against hippocampal neuronal apoptosis in diabetic encephalopathy by inhibiting JNK/MLCK signaling.
复制标题
绿茶通过抑制JNK/MLCK信号通路抑制糖尿病脑病海马神经元凋亡。
DOI：
10.3892/mmr.2021.12214
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发表时间：
2021-08
影响因子：
3.4
通讯作者：
Pan W
中科院分区：
文献类型：
--
作者：
Xu Y;Liu S;Zhu L;Dai L;Qian W;Zhang J;Li X;Pan W
虽然糖尿病脑病(DE)是糖尿病的主要晚期并发症，但DE姿势不稳的病理生理学机制仍知之甚少。已有研究表明，神经元凋亡与认知功能密切相关，但其机制尚不清楚。绿茶是一种非发酵茶，含有茶多酚、生物碱、氨基酸、多糖等多种成分。一些研究发现，喝绿茶可以降低神经退行性疾病的发病率，改善认知功能障碍。我们先前发现肌球蛋白轻链激酶(MLCK)调节高糖诱导的海马神经元的凋亡。在包括阿尔茨海默病和帕金森病在内的神经退行性疾病中，JNK信号通路的激活促进了神经元的凋亡。然而，JNK与MLCK之间的关系尚不清楚。用血清药理学方法获得绿茶血清，并将其应用于海马神经元。另外，建立1型糖尿病大鼠模型，给予绿茶提取物，采用Morris水迷宫实验、细胞计数试剂盒-8法、流式细胞术、免疫印迹和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测绿茶对糖尿病大鼠海马神经细胞凋亡的影响。结果表明，绿茶可通过抑制JNK/MLCK通路保护海马神经细胞的凋亡，最终改善糖尿病大鼠的认知功能。这项研究为绿茶的神经保护作用提供了新的见解。
Although diabetic encephalopathy (DE) is a major late complication of diabetes, the pathophysiology of postural instability in DE remains poorly understood. Prior studies have suggested that neuronal apoptosis is closely associated with cognitive function, but the mechanism remains to be elucidated. Green tea, which is a non-fermented tea, contains a number of tea polyphenols, alkaloids, amino acids, polysaccharides and other components. Some studies have found that drinking green tea can reduce the incidence of neurodegenerative diseases and improve cognitive dysfunction. We previously found that myosin light chain kinase (MLCK) regulates apoptosis in high glucose-induced hippocampal neurons. In neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease, activation of the JNK signaling pathway promotes neuronal apoptosis. However, the relationship between JNK and MLCK remains to be elucidated. Green tea serum was obtained using seropharmacological methods and applied to hippocampal neurons. In addition, a type 1 diabetes rat model was established and green tea extract was administered, and the Morris water maze test, Cell Counting Kit-8 assays, flow cytometry, western blotting and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling assays were used to examine the effects of green tea on hippocampal neuronal apoptosis in diabetic rats. The results demonstrated that green tea can protect against hippocampal neuronal apoptosis by inhibiting the JNK/MLCK pathway and ultimately improves cognitive function in diabetic rats. The present study provided novel insights into the neuroprotective effects of green tea.