Vaccine-induced immunity in baboons by using DNA and replication-incompetent adenovirus type 5 vectors expressing a human immunodeficiency virus type 1 gag gene
Vaccine-induced immunity in baboons by using DNA and replication-incompetent adenovirus type 5 vectors expressing a human immunodeficiency virus type 1 gag gene
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DOI：
10.1128/jvi.77.13.7663-7668.2003
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发表时间：
2003-07-01
影响因子：
5.4
通讯作者：
Shiver, JW
中科院分区：
文献类型：
--
作者：
Casimiro, DR;Tang, AM;Shiver, JW
在狒狒中检查了配方质粒DNA的细胞免疫原性和复制缺陷的人腺病毒，血清型5（AD5）疫苗载体，表达表达密码子优化的人类免疫缺陷病毒1型GAG基因的疫苗。 AD5疫苗能够诱导始终如一的强，长寿命的CD8（+） - 偏置的T细胞反应和体外细胞毒性活性。 DNA疫苗吸收的免疫反应比AD5疫苗引起的疫苗弱和高度可变。化学佐剂的制剂导致插孔特异性T细胞水平中等增加。通过助推器剂量的AD5或改性疫苗的Ankara疫苗增加DNA引发的反应表明细胞毒性和辅助剂反应的相对水平有所不同。讨论了这些结果的含义。
The cellular immunogenicity of formulated plasmid DNA and replication-defective human adenovirus, serotype 5 (Ad5) vaccine vectors expressing a codon-optimized human immunodeficiency virus type 1 gag gene was examined in baboons. The Ad5 vaccine was capable of inducing consistently strong, long-lived CD8(+)-biased T-cell responses and in vitro cytotoxic activities. The DNA vaccine-elicited immune responses were weaker than those elicited by the Ad5 vaccine and highly variable; formulation with chemical adjuvants led to moderate increases in the levels of Gag-specific T cells. Increasing the DNA-primed responses with booster doses of either Ad5 or modified vaccinia virus Ankara vaccines suggests a difference in the relative levels of cytotoxic and helper responses. The implications of these results are discussed.