A python script to design site-directed mutagenesis primers
A python script to design site-directed mutagenesis primers
复制标题
用于设计定点诱变引物的 python 脚本
DOI：
10.1002/pro.3806
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发表时间：
2020
期刊：
影响因子：
8
通讯作者：
Lin Shiqiang
中科院分区：
文献类型：
--
作者：
Bi Chuyun;Huang Xiaofang;Tang Dan;Shi Yuanyuan;Zhou Lixiang;Hu Yunzhuo;Chen Xuanyang;Qi Shiqian;Lin Shiqiang
引物设计是蛋白质研究中进行全质粒定点诱变的关键。传统的诱变引物都是人工设计的，既耗时又容易出错。在这里，我们提供了一个Python脚本，通过预设核苷酸组成和鸟嘌呤-胞嘧啶百分比（GC含量）的参数来搜索引物。运行结果表明，该脚本能够自动搜索到目标残基突变的引物。该脚本可以为全质粒位点定向突变的引物设计提供便利，并有助于蛋白质突变体的构建。
Primer design is essential to conduct whole plasmid site‐directed mutagenesis for protein study. Traditionally, primers of mutagenesis are designed manually that is time‐consuming and fallible. Here, we present a Python script for searching primers by presetting parameters of nucleotide composition and percentage of guanine‐cytosine (GC content). The running results showed that the script is able to search primers with mutations of target residue automatically. This script may facilitate primer design for whole plasmid site‐directed mutagenesis and aid protein mutant construction.