ENZYMATIC-PROPERTIES OF HUMAN HAGEMAN-FACTOR FRAGMENT WITH PLASMA PREKALLIKREIN AND SYNTHETIC SUBSTRATES
ENZYMATIC-PROPERTIES OF HUMAN HAGEMAN-FACTOR FRAGMENT WITH PLASMA PREKALLIKREIN AND SYNTHETIC SUBSTRATES
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DOI：
10.1016/0049-3848(80)90337-0
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发表时间：
1980-01-01
影响因子：
7.5
通讯作者：
PISANO, JJ
中科院分区：
文献类型：
--
作者：
HOJIMA, Y;TANKERSLEY, DL;PISANO, JJ
从丙酮处理的人血浆中分离出Hageman因子片段（HFf）。用天然底物、血浆前激肽释放酶和34种合成酰胺和酯测定了HFf的Km和kcat [催化常数]。最高的催化效率，kcat/Km，被发现与前激肽释放酶（2980 mM-1）。在合成底物中，D-Pro-Phe-Arg-pNA、Ac-Phe-Arg-OEt、Cbz-Arg-OMe和Bz-Ile-Glu-Gly-Arg-pNA具有4个最大的kcat/Km值（130-670 mM-1 s-1）。
Hageman factor fragment (HFf) was isolated from acetone-treated human plasma. The Km and kcat [catalytic constant] of HFf were determined with a natural substrate, plasma prekallikrein, and 34 synthetic amides and esters. The highest catalytic efficiency, kcat/Km, was found with prekallikrein (2980 mM-1). Of the synthetic substrates, D-Pro-Phe-Arg-pNA, Ac-Phe-Arg-OEt, Cbz-Arg-OMe and Bz-Ile-Glu-Gly-Arg-pNA had the 4 largest kcat/Km values (130-670 mM-1s-1).