Improvement in early diagnosis of Japanese spotted fever by using a novel Rick PCR system.
Improvement in early diagnosis of Japanese spotted fever by using a novel Rick PCR system.
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使用新型 Rick PCR 系统改进日本斑疹热的早期诊断。
DOI：
10.1111/1346-8138.13015
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发表时间：
2015
期刊：
影响因子：
3.1
通讯作者：
Mizutani H.
中科院分区：
文献类型：
--
作者：
Kondo M;Akachi S;Kawano M;Yamanaka K;Yamagiwa A;Gabazza EC;Ando K;Mizutani H.
立克次体疾病，包括日本斑点热(JSF)，是严重的传染病。延迟诊断有时会导致危及生命的肝脏疾病和弥漫性血管内凝血(DIC)。由于潜伏期短，血清学诊断对JSF的早期诊断并不可取。到目前为止，基于聚合酶链式反应(PCR)的诊断方法被用于早期诊断，如我们之前报道的那样，使用皮肤活检样本的敏感性高达90%。另一方面，同样的PCR方法使用血液样本的灵敏度被限制在50%以下。在本研究中，利用外周血样本，我们建立了一种新的诊断JSF的方法，该方法使用原始的聚合酶链式反应(Rick)系统，与传统的套式聚合酶链式反应相比具有更高的灵敏度。为立克次体感染的早期、敏感诊断提供了一种较好的诊断工具。
Rickettsiadiseases, including Japanese spotted fever (JSF), are serious infections. Delayed diagnosis occasionally results in life‐threatening liver disorders and disseminated intravascular coagulation (DIC). Because of the shortness of the latent period, serological diagnosis is not preferable for early diagnosis of JSF. Until now, a polymerase chain reaction (PCR)‐based diagnosis method has been used for early diagnosis, and the sensitivity reaches as high as 90% using skin biopsy samples as we previously reported. On the other hand, the sensitivity of the same PCR method using blood samples is limited at less than 50%. In the present study, using peripheral blood samples, we developed a novel diagnostic method for JSF using a Rick PCR system with original PCR primers, showing improved sensitivity compared with the conventional nested PCR. It may constitute a preferable diagnostic tool for early and sensitive diagnosis ofRickettsiainfection.