CXCR3 Binding Chemokine and TNFSF14 Over Expression in Bladder Urothelium of Patients With Ulcerative Interstitial Cystitis
CXCR3 Binding Chemokine and TNFSF14 Over Expression in Bladder Urothelium of Patients With Ulcerative Interstitial Cystitis
复制标题
DOI：
10.1016/j.juro.2009.11.007
复制
发表时间：
2010-03-01
影响因子：
6.6
通讯作者：
Nishizawa, Osamu
中科院分区：
文献类型：
--
作者：
Ogawa, Teruyuki;Homma, Toshiki;Nishizawa, Osamu
目的：我们调查的基因负责溃疡性间质性膀胱炎的DNA微阵列分析和定量实时聚合酶链reaction.Materials和方法：膀胱尿路上皮组织取自一个网站除了溃疡性病变的9例溃疡性间质性膀胱炎和9个对照组，包括7膀胱癌和2良性前列腺增生症的正常外观区域。从膀胱样品中提取总RNA，并使用全人类基因组DNA微阵列44 K（Agilent Technologies，Santa Clara，加州）比较这2组之间的基因表达。微阵列数据通过GeneSpring（TM）GX软件和Incidity（R）Pathway Analysis进行分析。结果：我们鉴定了564个探针，它们在mRNA中的表达量是对照组的4倍以上（p < 0.05），其中564个探针在mRNA中的表达量是对照组的4倍以上（p < 0.05）。
Purpose: We investigated the genes responsible for ulcerative interstitial cystitis by DNA microarray analysis and quantitative real-time polymerase chain reaction.Materials and Methods: Bladder urothelial tissues were taken from a site apart from the ulcerative lesion in 9 patients with ulcerative interstitial cystitis and from a normal-looking area in 9 controls, including 7 with bladder carcinoma and 2 with benign prostatic hyperplasia. Total RNA was extracted from bladder samples and gene expression was compared between these 2 groups using Whole Human Genome DNA microarray 44K (Agilent Technologies, Santa Clara, California). Microarray data were analyzed by GeneSpring(TM) GX software and Ingenuity(R) Pathway Analysis. Chosen genes were confirmed for altered transcription by quantitative real-time polymerase chain reaction.Results: We identified 564 probes that were significantly expressed in mRNA more than 4-fold vs those in controls using volcano plot analysis (p