[Study on proliferating potential by Ag-NORs staining in human pancreatic carcinoma].
[Study on proliferating potential by Ag-NORs staining in human pancreatic carcinoma].
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Ag-NORs染色对人胰腺癌增殖能力的研究
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
G. Asano
中科院分区：
文献类型：
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作者：
T. Aimoto;M. Onda;E. Uchida;G. Asano
NOR（核仁组织区）是编码核糖体 RNA 的 DNA 环，反映细胞活动。 Ag-NORs 染色适用于 10 个正常胰腺、10 个慢性胰腺炎和 23 个胰腺癌、17 个胃癌和 18 个结肠癌的石蜡切片。我们计算了平均 Ag-NORs 数量和 Ag-NORs 指数，并定义了 T/N 比。将平均 Ag-NOR 数量与胰腺癌中流式细胞术 (FCM) 获得的数据进行比较。胰腺良性组织（正常：1.86 +/- 0.38，胰腺炎：2.14 +/- 0.53）和恶性组织（4.02 +/- 1.19）之间 Ag-NORs 指数存在显着差异（p < 0.01）。与FCM数据相比，胰腺癌中平均Ag-NORs数量与S期细胞百分比之间存在显着相关性（r = 0.59，p < 0.01）。胰腺癌Ag-NORs指数显着低于胃癌（6.35±1.32）和结肠癌（7.66±1.35），但T/N比值无差异。总之，Ag-NORs 染色可用于分析胰腺癌的增殖活性。胰腺癌的增殖潜力低于胃癌和结肠癌。这可能是由于产生胰腺癌的背景粘膜的增殖潜力低。
NORs (Nucleolar Organizer Regions) are loops of DNA encoding ribosomal RNA, which reflect cellular activity. The Ag-NORs staining was applied to paraffin sections of 10 normal pancreas, 10 chronic pancreatitis and 23 pancreatic cancer, with 17 gastric and 18 colonic cancers. We calculated the mean Ag-NORs number and Ag-NORs index, and defined T/N ratio. The mean Ag-NORs number was compared with the data obtained by Flow cytometry (FCM) in pancreatic carcinoma. A significant difference in Ag-NORs index was found between benign (normal: 1.86 +/- 0.38, pancreatitis: 2.14 +/- 0.53) and malignant tissues (4.02 +/- 1.19) of the pancreas (p < 0.01). Compared with the data by FCM, there was a significant correlation between the mean Ag-NORs number and the percentage of S-phase cells in pancreatic carcinoma (r = 0.59, p < 0.01). Ag-NORs index in pancreatic carcinoma showed significantly lower value than that in cancer of stomach (6.35 +/- 1.32) and colon (7.66 +/- 1.35), but there was no difference in T/N ratio among them. In conclusion, the Ag-NORs staining is useful for analyzing proliferating activity in pancreatic carcinoma. The proliferating potential in pancreatic carcinoma is lower than that in gastric and colonic ones. This may be due to the low proliferating potential of the background mucosa from which pancreatic carcinoma will arise.