Detachment and killing of Aggregatibacter actinomycetemcomitans biofilms by dispersin B and SDS
Detachment and killing of Aggregatibacter actinomycetemcomitans biofilms by dispersin B and SDS
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DOI：
10.1177/154405910708600707
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发表时间：
2007-07-01
影响因子：
7.6
通讯作者：
Kaplan, J. B.
中科院分区：
文献类型：
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作者：
Izano, E. A.;Wang, H.;Kaplan, J. B.
牙周病原聚集菌放线菌在体外非生物表面形成坚韧的生物膜。本研究的目的是测定放线菌生物膜对阴离子表面活性剂十二烷基硫酸钠（SDS）剥离和杀伤的敏感性。我们发现由野生型菌株形成的生物膜对SDS剥离具有抗性。相反，由缺乏PGA（聚n -乙酰氨基葡萄糖）（一种生物膜基质多糖）生产的等基因突变菌株形成的生物膜对SDS剥离敏感。用分散酶B（一种pga降解酶）对野生型生物膜进行预处理，使其对SDS剥离敏感，SDS介导的细胞杀伤增加了约99%。我们得出结论，PGA可以保护A. actio -mycetemcomitans细胞免受SDS的分离和杀伤。分散素B和SDS可能是治疗由放线菌和其他产生pga的细菌引起的慢性感染的有效药物。
The periodontopathogen Aggregatibacter actinomycetemcomitans forms tenacious biofilms on abiotic surfaces in vitro. The objective of the present study was to measure the susceptibility of A. actinomycetemcomitans biofilms to detachment and killing by the anionic surfactant sodium dodecyl sulfate (SDS). We found that biofilms formed by a wild-type strain were resistant to detachment by SDS. In contrast, biofilms formed by an isogenic mutant strain that was deficient in the production of PGA (poly-N-acetyl-glucosamine), a biofilm matrix polysaccharide, were sensitive to detachment by SDS. Pretreatment of wild-type biofilms with dispersin B, a PGA-degrading enzyme, rendered them sensitive to detachment by SDS and resulted in a > 99% increase in SDS-mediated cell killing. We concluded that PGA protects A. actino-mycetemcomitans cells from detachment and killing by SDS. Dispersin B and SDS may be useful agents for treating chronic infections caused by A. actinomycetemcomitans and other PGA-producing bacteria.