The development of chemosensitivity in extrasynaptic areas of the neuronal surface after denervation of parasympathetic ganglion cells in the heart of the frog
The development of chemosensitivity in extrasynaptic areas of the neuronal surface after denervation of parasympathetic ganglion cells in the heart of the frog
复制标题
青蛙心脏副交感神经节细胞去神经后神经元表面突触外区域化学敏感性的发展
DOI：
10.1098/rspb.1971.0047
复制
发表时间：
1971
期刊：
影响因子：
--
通讯作者：
A. Harris
中科院分区：
文献类型：
--
作者：
S. W. Kuffler;M. Dennis;A. Harris
1.支配青蛙心脏的迷走交感神经干在两侧被切断。2至28天后，这失神经支配的化学敏感性的失神经元的表面进行了探讨与ACh的离子电渗微应用到限制区域。2.而在正常神经支配的神经元中，突触区域单独地是高度化学敏感的，在去神经支配后，整个细胞表面变得对ACh敏感。突触传递在去神经支配后的第二天（30至40小时）失败（青蛙保持在22至24 ° C），同时新的化学感受区开始出现。在4至8天后，这种化学敏感性的发展达到峰值，并保持在相同水平4周（本试验的最长时间）。3.比较了不同动物不同细胞的化疗敏感性测量结果。在正常神经支配的神经元的突触区的精细本地化的化学敏感性是类似的幅度均匀分布的敏感性在失神经支配的神经元。
1. The vagosympathetic trunks innervating the heart of the frog were cut on both sides. Two to 28 days following this denervation the chemosensitivity of the surface of the denervated neurons was explored with iontophoretic microapplication of ACh to restricted areas. 2. While in normally innervated neurons the synaptic areas alone are highly chemosensitive, after denervation the whole cell surface becomes sensitive to ACh. Synaptic transmission fails on the second day (30 to 40 h) after denervation (in frogs kept at 22 to 24 °C) and at the same time new chemoreceptive areas start to appear. After 4 to 8 days this development of chemosensitivity has reached a peak and remains at the same level for 4 weeks (the longest period of the present tests). 3. Measurements of chemosensitivity from different cells in different animals were compared. The finely localized chemosensitivity at synaptic areas in normally innervated neurons was of similar magnitude as the uniformly distributed sensitivity in denervated neurons.