Effects of the natural and artificial menstrual cycle on the production of osteoprotegerin and the bone resorptive cytokines IL-1β and IL-6
Effects of the natural and artificial menstrual cycle on the production of osteoprotegerin and the bone resorptive cytokines IL-1β and IL-6
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DOI：
10.1007/s00223-002-2037-y
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发表时间：
2003-01-01
影响因子：
4.2
通讯作者：
Beck-Nielsen, H
中科院分区：
文献类型：
--
作者：
Abrahamsen, B;Stilgren, LS;Beck-Nielsen, H
月经周期内骨重建的变化。虽然黄体期是伴随着。通过减少骨吸收，这也是一个矛盾的时间增加生产的骨吸收细胞因子。本研究检验了月经周期内血清骨保护素（OPG）的变化阻止骨重建增加的假设，否则骨重建将是黄体产生再吸收细胞因子的能力增加的结果。研究人群由健康女性志愿者组成：绝经前女性（n = 11，平均年龄39.4岁± 6.1岁），无周期紊乱。绝经后妇女（n = 11，平均年龄56.8 y +/- 3.6）接受周期性HRT（雌二醇和醋酸炔诺酮）。将黄体期和卵泡期血液样品稀释并在有和没有脂多糖（LPS）的情况下培养24小时。通过ELISA测定上清液中的IL-1 β和IL-6。ELISA法测定血清OPG。LPS刺激的IL-1 β和IL-6的产生在黄体期显著升高。当分析仅限于自然月经周期时，只有IL-1产生的增加仍然具有统计学意义。NTX排泄量在HRT的两个阶段相似，但在绝经前妇女的黄体期无显著性降低（p = 0.05）。OPG水平没有表现出任何月经周期依赖性变化。总之，骨吸收在黄体期通过一种不涉及血清OPG升高的机制受到抑制。血细胞的细胞因子分泌能力增加可能是黄体期特有的附带现象，但与骨代谢无关。
Bone remodelling changes within the menstrual cycle. Though the luteal phase is accompanied. by decreased bone resorption, it is also paradoxically a time of increased production of bone resorptive cytokines. The present study examined the hypothesis that changes in serum osteoprotegerin (OPG) within the menstrual cycle prevent the increase in bone remodelling, which would otherwise have been the result of the luteal increase in the capacity for producing resorptive cytokines. The study population consisted of healthy female volunteers: premenopausal women (n = 11, mean age 39.4 y +/- 6.1) without cycle irregularities. Postmenopausal women (n = 11, mean age 56.8 y +/- 3.6) receiving cyclic HRT (estradiol and noretisterone acetate). Luteal and follicular phase blood samples were diluted and cultured for 24 hours with and without lipopolysaccharide (LPS). The supernatant was assayed for IL-1beta and IL-6 by ELISA. Serum OPG was measured by ELISA. The LPS-stimulated production of IL-1beta and IL-6 was significantly higher in the luteal phase. When the analysis was restricted to the natural menstrual cycle, only the increase in IL-1 production remained statistically significant. NTX excretion was similar in the two phases of HRT, but decreased nonsignificantly (p = 0.05) in the luteal phase in the premenopausal women. OPG levels did not exhibit any menstrual cycle-dependent changes. In conclusion, bone resorption is suppressed in the luteal phase through a mechanism that does not involve increases in serum OPG. An increased cytokine secretory capacity of blood cells may be an epiphenomenon particular to the luteal phase but unrelated to bone metabolism.