Local immune responses to two stages of Ichthiophthirius multifiliis in ginbuna crucian carp
Local immune responses to two stages of Ichthiophthirius multifiliis in ginbuna crucian carp
复制标题
银鲫对两阶段多子虫的局部免疫反应
DOI：
10.1016/j.fsi.2021.08.013
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发表时间：
2021
影响因子：
4.7
通讯作者：
Somamoto T
中科院分区：
文献类型：
--
作者：
Shiota K;Sukeda M;Prakash H;Kondo M;Nakanishi T;Nagasawa T;Nakao M;Somamoto T
多丝小瓜虫是一种纤毛原生动物寄生虫，已知可感染许多淡水硬骨鱼。上皮组织是寄生虫渗透和定居的地方，表征上皮组织中的免疫系统是理解宿主-寄生虫相互作用的关键。本研究采用鱼鳍内给药法检测了寄生虫感染期（幼虫期和滋养期）的体内局部免疫反应，该方法已被开发用于分析鱼鳍体内免疫反应。在注射抗原的鳍腔中，CD 8 α+和CD 4 + T细胞比例显著增加。加塔-3和T-bet mRNA的表达，调节辅助性T细胞的分化，从营养体抗原注射部位的白细胞中显著上调。相比之下，注射部位的先天免疫成分巨噬细胞和中性粒细胞的百分比显着下降。这些结果表明，I.多丝抗原抑制巨噬细胞和嗜中性粒细胞的迁移，T细胞是I的第一反应者。多丝的因此，为了更好地了解宿主免疫与I.因此，今后的研究重点应放在探讨I. multifiliisor检查硬骨鱼T细胞的先天功能。
Ichthyophthirius multifiliis is a ciliated protozoan parasite and is known to infect many freshwater teleosts. Characterizing the immune system in epithelial tissues, where the parasites penetrate and settle, is key to understanding host–parasite interactions. This study examined local immune responsesin vivoto the infective stage (theront and trophont) of the parasites using intra-fin administration, which has been developed to analyzein vivoimmune responses using fish fin. CD8α+and CD4+T-cell compositions were increased significantly in the fin cavity injected with theront or trophont antigens. The expression of GATA-3 and T-bet mRNA, which regulate differentiation of helper T-cells, was upregulated significantly in leukocytes from the trophont antigen–injected site. In contrast, the percentages of macrophages and neutrophils, which are innate immunity components, were decreased significantly in the injection sites. These results suggest thatI. multifiliisantigens inhibit the migration of macrophages and neutrophils, and T-cells are the first responders toI. multifiliis. Thus, to better understand the interaction of host immunity andI. multifiliis, further studies should focus on exploring the inhibitory factors fromI. multifiliisor examining innate functions of teleost T-cells.