The adhesion of putative probiotic lactobacilli to cultured epithelial cells and porcine intestinal mucus
The adhesion of putative probiotic lactobacilli to cultured epithelial cells and porcine intestinal mucus
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DOI：
10.1111/j.1365-2672.2007.03636.x
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发表时间：
2008-04-01
影响因子：
4
通讯作者：
Qiao, S. Y.
中科院分区：
文献类型：
--
作者：
Li, X. J.;Yue, L. Y.;Qiao, S. Y.
目的：探讨乳酸杆菌的粘附和随后的竞争排斥能力对pathogenes.Methods和结果：四种假定的益生乳酸杆菌的粘附能力进行了研究。首先，通过光学和电子显微镜检查对Caco-2细胞的粘附。然后用[甲基-H-3]胸苷标记这四种物质，并通过放射性测定它们对猪肠粘液的粘附。与十二指肠和空肠粘液相比，测试的乳酸杆菌显示出最好的粘附在回肠粘液上。氧化化合物预处理（NaIO 3和NaIO 4）显著降低了乳酸杆菌对粘液的粘附。用蛋白水解酶（蛋白酶K和胰蛋白酶）预处理粘液导致罗伊氏乳杆菌血清型I2021的粘附增加，但其他物种的结果是可变的。乳酸杆菌血清型发酵I5007表现出最大的粘附潜力，并发挥最好的竞争性排除沙门氏菌和Escherichias.Conclusions：粘附能力的乳酸杆菌是种特异性。粘附指数越高的乳酸杆菌竞争排斥能力越强。研究意义和影响：本研究提示乳酸杆菌的粘附性与竞争排斥能力之间存在正相关关系。此外，体外粘附试验是筛选未知乳酸杆菌的可行方法，具有潜在的工业应用前景。
Aims: To investigate the adhesion of lactobacilli and their subsequent competitive exclusion ability against pathogens.Methods and Results: Four species of putative probiotic lactobacilli were studied for their adhesion abilities. First, the adhesion to Caco-2 cells was examined by light and electron microscopy. The four species were then labelled by [methyl-H-3] thymidine and their adhesion to porcine intestinal mucus was determined by radioactivity. The tested lactobacilli showed best adhesion on ileal mucus compared with duodenal and jujenal mucus. Oxidative compound pre-treatment (NaIO3 and NaIO4) dramatically decreased the adhesion of the lactobacilli to mucus. Pre-treating mucus with proteolytic enzymes (proteinase K and trypsin) resulted in the increase of adhesion in Lactobacillus serotype Reuteri I2021, but the results in the other species were variable. Lactobacillus serotype Fermentum I5007 showed greatest adhesion potential and exerted the best competitive exclusion against Salmonella and Escherichia.Conclusions: Adhesion ability in lactobacilli is species-specific. Lactobacilli with higher adhesion index have better competitive exclusion ability.Significance and Impact of the Study: This study suggests that there is a positive correlation between adhesion and competitive exclusion ability of lactobacilli. Additionally, the in vitro adhesion assay is a feasible way to screen unknown lactobacilli, potentially for future industrial applications.