Effect of fluoride on artificial caries lesion progression and repair in human enamel:: Regulation of mineral deposition and dissolution under in vivo-like conditions
Effect of fluoride on artificial caries lesion progression and repair in human enamel:: Regulation of mineral deposition and dissolution under in vivo-like conditions
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DOI：
10.1016/j.archoralbio.2006.08.012
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发表时间：
2007-02-01
影响因子：
3
通讯作者：
Margolis, Henry C.
中科院分区：
文献类型：
--
作者：
Yamazaki, Hajime;Litman, Amy;Margolis, Henry C.
目的：本研究在体外测定了在相关口腔条件下氟化物对（1）健康人牙釉质脱矿和（2）人工龋样病变进展的影响。方法：将健康人牙釉质的薄切片暴露于相对于牙釉质不饱和的溶液中，其程度类似于体内蔗糖暴露后牙菌斑液中发现的程度，含有在牙斑液中发现的氟化物浓度（0- 0.38ppm）。使用定量显微放射照相术监测矿物质变化98天。氟化物（1.0-25.0 ppm）对人工龋样病变进展的影响进行了类似的studied.Results：氟浓度为0.19 ppm和更大的发现，以防止脱矿的健康釉质在体外。然而，需要显著更高浓度的氟化物（25.0 ppm）来防止人工龋样病变的进一步脱矿。具有中等氟化物浓度（2.1-10.1 ppm）的脱矿溶液同时诱导病变外部的矿化和内部的脱矿。羟基磷灰石pellet.Conclusions：我们的研究结果表明，所观察到的氟对釉质脱矿的影响不仅是一个函数的散装溶液的性能，但也取决于龋状态的釉质表面。一个机械模型表明，在比较健全的釉质表面，需要更高浓度的氟化物，以防止在体内的条件下，因为矿物离子的扩散，促进矿化的进展，促进人工龋样病变的速度限制。(c)2006爱思唯尔有限公司保留所有权利。
Objective: This study was carried out to determine in vitro the effect of fluoride on (1) the demineralization of sound human enamel and (2) the progression of artificial caries-like lesions, under relevant oral conditions.Methods: Thin sections of sound human enamel were exposed to solutions undersaturated with respect to tooth enamel to a degree similar to that found in dental plaque fluid following sucrose exposure in vivo, containing fluoride concentrations (0-0.38 ppm) found in plaque fluid. Mineral changes were monitored for 98 days, using quantitative microradiography. The effect of fluoride (1.0-25.0 ppm) on the progression of artificial caries-like lesions was similarly studied.Results: Fluoride concentrations of 0.19 ppm and greater were found to prevent the demineralization of sound enamel in vitro. However, significantly higher concentrations of fluoride (25.0 ppm) were required to prevent further dernineralization of artificial caries-like lesions. Demineralizing solutions with intermediate fluoride concentrations (2.1-10.1 ppm) induced simultaneously remineralization in the outer portion of the lesion and dernineralization in the inner portion. Simultaneous remineralization and demineralization were also observed in hydroxyapatite pellets.Conclusions: Our results show that the observed effect of fluoride on enamel demineralization is not solely a function of bulk solution properties, but also depends on the caries-status of the enamel surface. A mechanistic model presented indicates that, in comparison to sound enamel surfaces, higher concentrations of fluoride are required to prevent the progression of artificial caries-like lesions under in vivo-like conditions since the diffusion of mineral ions that promote remineralization is rate-limiting. (c) 2006 Elsevier Ltd. All rights reserved.