The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava
The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava
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分子伴侣MeHSP90招募MeWRKY20和Me过氧化氢酶1以调节木薯的抗旱性
DOI：
10.1111/nph.16346
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发表时间：
2020-01-02
期刊：
影响因子：
9.4
通讯作者：
Shi, Haitao
中科院分区：
文献类型：
--
作者：
Wei, Yunxie;Liu, Wen;Shi, Haitao
90 kDa 热休克蛋白 (HSP90) 广泛参与植物的各种发育过程和逆境响应。然而，分子伴侣 HSP90 构建的蛋白质复合物及其在木薯中的功能仍然难以捉摸。在这项研究中，我们报告说，HSP90 通过使用 HSP90 的两种特定蛋白抑制剂（格尔德霉素 (GDA) 和根赤霉素 (RAD)）调节脱落酸 (ABA) 和过氧化氢 (H2O2)，对于木薯的干旱胁迫抗性至关重要。在 10 个 MeHSP90 中，MeHSP90.9 的转录主要在干旱胁迫期间被诱导。进一步研究确定 MeWRKY20 和 MeCatalase1 是 MeHSP90.9 相互作用蛋白。通过病毒诱导的基因沉默，MeHSP90.9、MeWRKY20 或 MeCatalase1 沉默的植物在木薯中表现出干旱敏感性，表明它们对干旱胁迫响应很重要。 MeHSP90.9可以促进MeNCED5启动子W盒元件上MeWRKY20的直接转录激活，MeNCED5启动子编码ABA生物合成中的关键酶。此外，MeHSP90.9 正向调节 MeCatalase1 的活性，并且 MeHSP90.9 沉默的木薯叶片在干旱胁迫下积累更多的 H2O2。综上所述，我们证明 MeHSP90.9 伴侣复合物是木薯抗旱胁迫性的调节剂。
The 90 kDa heat shock protein (HSP90) is widely involved in various developmental processes and stress responses in plants. However, the molecular chaperone HSP90-constructed protein complex and its function in cassava remain elusive. In this study, we report that HSP90 is essential for drought stress resistance in cassava by regulating abscisic acid (ABA) and hydrogen peroxide (H2O2) using two specific protein inhibitors of HSP90 (geldanamycin (GDA) and radicicol (RAD)). Among 10 MeHSP90s, the transcript of MeHSP90.9 is largely induced during drought stress. Further investigation identifies MeWRKY20 and MeCatalase1 as MeHSP90.9-interacting proteins. MeHSP90.9-, MeWRKY20-, or MeCatalase1-silenced plants through virus-induced gene silencing display drought sensitivity in cassava, indicating that they are important to drought stress response. MeHSP90.9 can promote the direct transcriptional activation of MeWRKY20 on the W-box element of MeNCED5 promoter, encoding a key enzyme in ABA biosynthesis. Moreover, MeHSP90.9 positively regulates the activity of MeCatalase1, and MeHSP90.9-silenced cassava leaves accumulate more H2O2 under drought stress. Taken together, we demonstrate that the MeHSP90.9 chaperone complex is a regulator of drought stress resistance in cassava.