Acute Swine Model for Assessing Biocompatibility of Biomedical Interface Materials.
Acute Swine Model for Assessing Biocompatibility of Biomedical Interface Materials.
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用于评估生物医学界面材料生物相容性的急性猪模型。
DOI：
10.1089/ten.tec.2017.0310
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Fukamachi,Kiyotaka
中科院分区：
文献类型：
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作者：
Takaseya,Tohru;Fumoto,Hideyuki;Zhu,Junmin;Shiose,Akira;Kobayashi,Mariko;Fujiki,Masako;Arakawa,Yoko;Dessoffy,Raymond;KramerJr,LarryD;Fukamachi,Kiyotaka
我们建立了一个急性动物模型，用于对生物相容性材料界面进行早期、直接和可重复性的评估。12只猪建立了双侧股动脉-静脉分流管：每支分流管两根，左侧两根，右侧两根。我们评估了两组：不控制流量(UF；分流无调节)和控制流量(CF；分流∼50 毫升/分钟)。每例患者双侧分流1 h和3 h，分别于基线、1、3 h记录动脉血气和全血细胞计数，平均分流流量分别为5 32 ± 88 m L/m in UF和52 ± 8 m L/m in CF。Cf组(0.5 m L/m in；平均流量的1%)较UF组(24.8 m L/m in；平均流量的5%)的流量差异小得多。在UF中，从开始分流到1 h，pH发生了显著变化；从开始到3 h，PO2和PCO2发生了显著变化。这种采用双侧股动脉分流并控制血流的猪模型提供了一种可靠、重复性好、易于实施的方法，可以在研究的早期阶段评估装置涂层的生物相容性。
We established an acute animal model for early, straightforward, and reproducible assessment of a biocompatible material interface. Bilateral femoral artery-to-vein shunts were created in 12 pigs: two tubes per shunt, the left two coated and the right two uncoated. We evaluated two groups: uncontrolled flow (UF; shunt flow unregulated) and controlled flow (CF; shunt flow ∼50 mL/min). For each case on each side, two shunts were evaluated: one for 1 h and the other for 3 h. Arterial blood gas and complete blood count were recorded at baseline, 1, and 3 h. Mean shunt flows were 532 ± 88 mL/min UF and 52 ± 8 mL/min CF. Differences in flow were much smaller in CF (0.5 mL/min; 1% of mean flow) than UF (24.8 mL/min; 5% of mean flow). In UF, significant changes occurred: in pH, from start of shunting through 1 h; in pO2and pCO2, from start through 3 h. This swine model using bilateral femoral shunts with controlled blood flow provides a reliable, reproducible, easily implemented method by which to evaluate biocompatibility of device coatings at an early stage of investigation.