Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species
Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species
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DOI：
10.1111/j.1745-7254.2008.00878.x
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发表时间：
2008-11-01
影响因子：
8.2
通讯作者：
Hyun, Jin-won
中科院分区：
文献类型：
--
作者：
Kim, So-hyung;Kang, Kyoung-ah;Hyun, Jin-won
目的：目的：研究七叶壳（6，7-dihydro-xycoumarin，6，7-二氢香豆素）对H2 O2诱导的中国仓鼠肺成纤维细胞（V79-4）损伤的抗氧化作用。研究方法：以1，1-二苯基-2-苦肼基（DPPH）自由基、羟自由基和细胞内活性氧（ROS）为指标，测定其清除自由基的活性。此外，通过测定与硫代巴比妥酸反应的有关物质测定脂质过氧化作用。使用蛋白质羰基ELISA试剂盒测定蛋白质中羰基形成的量。同时，通过蛋白质印迹和免疫荧光图像检测细胞DNA损伤。通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物测定评估细胞活力。结果：七棵壳具有清除DPPH自由基、清除羟自由基和清除细胞内活性氧的活性。七叶壳酮具有清除自由基的作用，对H2 O2引起的细胞脂质过氧化、蛋白质羰基化和DNA损伤具有保护作用。因此，escuoke恢复了暴露于H（2）O（2）的细胞活力。结论：四角壳可通过其抗氧化作用有效减轻氧化应激引起的细胞损伤。因此，七叶壳可作为功能性食品和医药原料的开发利用。
Aim: To investigate the anti-oxidant properties of esculetin (6,7-dihydro-xycoumarin) against H(2)O(2)-induced Chinese hamster lung fibroblast (V79-4) damage. Methods: The radical scavenging activity was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immuno-fluorescence image. Cell viability was assessed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H(2)O(2). Therefore, esculetin recovered cell viability exposed to H(2)O(2). Conclusion: Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.