Sperm competition in the absence of fertilization in Caenorhabditis elegans.
Sperm competition in the absence of fertilization in Caenorhabditis elegans.
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发表时间：
1999-05
期刊：
影响因子：
3.3
通讯作者：
Andrew Singson;K. L. Hill;S. L'Hernault
中科院分区：
文献类型：
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作者：
Andrew Singson;K. L. Hill;S. L'Hernault
两性自花授粉是线虫的主要繁殖方式。然而，当两性人与男性杂交时，几乎所有的卵母细胞都是由男性来源的精子受精的。这种精子在生殖过程中的优先地位是由于男性来源精子的竞争优势，并导致两性自我生育的功能受到抑制。在这项研究中，让受精缺陷精子受精的突变雄性被用来揭示两性人内部的精子竞争不需要成功受精。然而，精子竞争确实需要正常的精子运动能力。此外，精子竞争不是一个绝对的过程，因为未由男性来源的精子受精的卵母细胞有时可以由两性人来源的精子受精。这些结果表明，异交后代是野生型杂交的结果，因为雄性来源的精子具有竞争性优势，两性来源的精子无法提供给卵母细胞。这项研究中描述的精子竞争分析将有助于进一步对目前发现的大量改变线虫精子功能和发育的突变进行分类。
Hermaphrodite self-fertilization is the primary mode of reproduction in the nematode Caenorhabditis elegans. However, when a hermaphrodite is crossed with a male, nearly all of the oocytes are fertilized by male-derived sperm. This sperm precedence during reproduction is due to the competitive superiority of male-derived sperm and results in a functional suppression of hermaphrodite self-fertility. In this study, mutant males that inseminate fertilization-defective sperm were used to reveal that sperm competition within a hermaphrodite does not require successful fertilization. However, sperm competition does require normal sperm motility. Additionally, sperm competition is not an absolute process because oocytes not fertilized by male-derived sperm can sometimes be fertilized by hermaphrodite-derived sperm. These results indicate that outcrossed progeny result from a wild-type cross because male-derived sperm are competitively superior and hermaphrodite-derived sperm become unavailable to oocytes. The sperm competition assays described in this study will be useful in further classifying the large number of currently identified mutations that alter sperm function and development in C. elegans.