Inhibition of pestivirus infection in cell culture by envelope proteins E-rns and E2 of classical swine fever virus: E-rns and E2 interact with different receptors
Inhibition of pestivirus infection in cell culture by envelope proteins E-rns and E2 of classical swine fever virus: E-rns and E2 interact with different receptors
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DOI：
10.1099/0022-1317-78-11-2779
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发表时间：
1997-11-01
影响因子：
3.8
通讯作者：
Moormann, RJM
中科院分区：
文献类型：
--
作者：
Hulst, MM;Moormann, RJM
用昆虫细胞合成的猪瘟病毒（CSFV）囊膜糖蛋白E-ms和E2纯品，研究了猪瘟病毒（CSFV）和牛病毒性腹泻病毒（BVDV）对猪和牛细胞的感染。100 μ g/ml的E-ms对CSFV感染猪肾细胞产生几乎100%的抑制。去除病毒后，在覆盖培养基（生长培养基）中不需要E-ms来维持这种抑制水平。相反，10 μ g/ml E2对CSFV感染猪肾细胞的100%抑制作用仅在将E2加入覆盖培养基中时达到。当省略E2时，达到最大50%的抑制。这表明，在病毒和E2从细胞中去除后，仍然通过仍然结合在细胞表面的病毒颗粒发生感染。用100 μ g/ml E-ms处理可从细胞表面释放这些颗粒。此外，E-ms结合不可逆的细胞表面易感或不易感瘟病毒感染和CSFV的细胞间传播完全抑制E2，但不E-ms。这些结果表明，E-ms和E2与不同的细胞表面受体相互作用。CSFV E2对BVDV感染猪和牛细胞的抑制表明CSFV E2和BVDV E2具有相同的受体。从猪分离的BVDV 5250株能被CSFV E-ms有效抑制，而从牛分离的几株则不能，这表明E-ms的构象在宿主嗜性中起作用。
Pure preparations of envelope glycoproteins E-ms and E2 of classical swine fever virus (CSFV) synthesized in insect cells were used to study infection of porcine and bovine cells with the pestiviruses CSFV and bovine viral diarrhoea virus (BVDV). Almost 100% inhibition of infection of porcine kidney cells with CSFV was produced by 100 mu g/ml E-ms. After removal of the virus no E-ms was needed in the overlay medium (growth medium) to maintain this level of inhibition. In contrast, 100% inhibition of infection of porcine kidney cells with CSFV by 10 mu g/ml E2 was only achieved when E2 was added to the overlay medium. When E2 was omitted, a maximum of 50% inhibition was achieved. This indicated that after the virus and E2 were removed from the cells, infection still occurred, by virus particles which were still bound to the cell surface. Treatment with 100 mu g/ml E-ms released these particles from the cell surface. Furthermore, E-ms bound irreversibly to the surface of cells susceptible or unsusceptible to pestivirus infection and cell-to-cell spread of CSFV was completely inhibited by E2 but not by E-ms. These results demonstrated that E-ms and E2 interacted with different cell surface receptors. Inhibition of BVDV infection of porcine and bovine cells by CSFV E2 suggested that CSFV E2 and BVDV E2 share an identical receptor. BVDV strain 5250 isolated from pigs was efficiently inhibited by CSFV E-ms, whereas several BVDV strains isolated from cattle were not, suggesting that the conformation of E-ms plays a role in host tropism.