Responsiveness to light of the circadian clock controlling eclosion in the blowfly, Lucilia cuprina
Responsiveness to light of the circadian clock controlling eclosion in the blowfly, Lucilia cuprina
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绿蝇绿蝇对控制羽化的生物钟的光响应
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发表时间：
1985
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通讯作者：
Peter H. Smith
中科院分区：
文献类型：
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作者：
Peter H. Smith
摘要。绿蝇（Lucilia cuprina，Wiedemann）在黎明时分羽化。羽化的节律在黑暗和高强度（490μW cm-2）的恒定光照下持续，周期接近24小时。控制羽化的生物钟对光的敏感性根据生命周期的阶段而变化很大。在幼虫生命期间，黑暗中的自由运行节律可以被100μW cm-2强度的光脉冲相移，从1型相位响应曲线过渡到0型，脉冲在1和8小时之间发生。将LD的最后光照期延长至24 h，然后恒定黑暗，将节律的相位重置12 h，从恒定光照到恒定黑暗的过渡启动了由保持在恒定光照下的成虫收集的卵饲养的果蝇的节律性。化蛹后，节律对光相对不敏感。节律性有时是由从恒定的光到恒定的黑暗的过渡引起的，但是在进入恒定的黑暗之前，通过延长LD的最后一个光周期，节律的相位不会发生变化。在化蛹后应用重复LD周期启动并引导节律。
ABSTRACT. Eclosion in Lucilia cuprina (Wiedemann) occurs near dawn. The rhythm of eclosion persists in both darkness and constant light of high intensity (490μW cm‐2) with a period close to 24h. The sensitivity to light of the circadian clock controlling eclosion varies greatly according to the stage of the life cycle. During larval life the free running rhythm in darkness can be phase shifted by light pulses of 100μW cm‐2 intensity, with the transition from a Type 1 phase response curve to a Type 0, occurring with pulses of between 1 and 8h. Extending the last light period of LD to 24 h followed by constant darkness resets the phase of the rhythm by 12h, a transition from constant light to constant darkness initiates rhythmicity in flies made arrhythmic by being reared from eggs collected from adults maintained in constant light. After pupariation, the rhythm is relatively insensitive to light. Rhythmicity is sometimes induced by a transition from constant light to constant darkness, but the phase of the rhythm is not shifted by extending the last light period of LD before entering constant darkness. Repeated LD cycles applied after pupariation initiate and entrain the rhythm.