Erythrocyte rheology.
Erythrocyte rheology.
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DOI：
10.1136/jcp.38.9.965
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发表时间：
1985-09
影响因子：
3.4
通讯作者：
J. Stuart
中科院分区：
文献类型：
--
作者：
J. Stuart
红细胞变形性以前是通过其对全血粘度的贡献来测量的。现在更常见的是通过过滤红细胞或将红细胞吸入直径为3-5微米的孔中，并通过使用激光衍射法测量剪切诱导的红细胞伸长来测量。红细胞过滤技术的最新改进包括从测试红细胞悬浮液中去除急性期反应物、超声波清洗和滤膜的重复使用、认识到平均细胞体积作为通过3微米直径孔的流量的决定因素的重要性以及检测变形较小的红细胞亚群的能力。使用Ektacytometer通过激光衍射法测量红细胞伸长也受细胞大小的影响，需要根据平均细胞体积进行校正。这些进展大大提高了流变学方法的灵敏度和特异性，用于测量红细胞的变形性和研究流变活性药物的作用模式。
Erythrocyte deformability was formerly measured by its contribution to whole blood viscosity. It is now more commonly measured by filtration of erythrocytes through, or aspiration into, pores of 3-5 microns diameter and by the measurement of shear induced erythrocyte elongation using laser diffractometry. Recent improvements in the technology for erythrocyte filtration have included the removal of acute phase reactants from test erythrocyte suspensions, ultrasonic cleaning and reuse of filter membranes, awareness of the importance of mean cell volume as a determinant of flow through 3 microns diameter pores, and the ability to detect subpopulations of less deformable erythrocytes. Measurements of erythrocyte elongation by laser diffractometry, using the Ektacytometer, are also influenced by cell size and need to be corrected for mean cell volume. These advances have greatly improved the sensitivity and specificity of rheological methods for measuring the deformability of erythrocytes and for investigating the mode of action of rheologically active drugs.